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Archives of Virology

, Volume 162, Issue 9, pp 2855–2860 | Cite as

Genetic diversity of human papillomavirus types 35, 45 and 58 in cervical cancer in Brazil

  • Diogo Lisbôa Basto
  • João Paulo Vidal
  • Valéria Barbosa Pontes
  • Shayany Pinto Felix
  • Laine Celestino Pinto
  • Bruno Moreira Soares
  • Luis Felipe Martins
  • Flávia Miranda Correa
  • Raquel Carvalho Montenegro
  • Cláudia Bessa Pereira Chaves
  • Liz Maria Almeida
  • Miguel Ângelo Martins MoreiraEmail author
Brief Report

Abstract

In Brazil, most studies of intra-type variants of human papillomavirus (HPV) have focused on HPV16 and HPV18, but other high-risk HPV types have not been studied. Here, we report the prevalence of lineages and variants of HPV35, HPV45 and HPV58 in cervical cancers from the Amazonian and Southeast Brazilian regions. The most frequent sublineages were A1 for HPV35, B2 for HPV45, and A2 for HPV58. The Southeast region had a higher frequency of the B2 sublineage of HPV45, and for HPV35, the genetic and nucleotide sequence diversity were higher in the Southeast region, suggesting that regional factors are influencing the diversity and lineage prevalence.

Keywords

Cervical cancer Papillomavirus HPV35 HPV45 HPV58 HPV diversity 

Notes

Acknowledgements

We want to thank the physicians who collected the tumor biopsies. This work was supported by the National Institute for Cancer Control (INCT do Cancer: http://www.inct-cancer-control.com.br/); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Grants 573806/2008-0, 484005/2013-8 and 305873/2014-8; Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) Grant E26/170.026/2008; Ministry of Health – Brazil; and Pan-American Health Organization (PAHO).

Compliance with ethical standards

Ethical approval

All procedures were approved by the Research Ethics Committees of the Instituto Nacional de Câncer (CAAE 53398416.0.0000.5274) and the Hospital Ophir Loyola (CAAE 03288212.0.1001.0018).

Informed consent

All patients signed an informed consent form.

Animal rights

This article does not contain any studies with animals.

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

705_2017_3439_MOESM1_ESM.tif (178 kb)
Haplotype network for HPV35 generated by median joining analysis, based on samples of Rio de Janeiro and Pará, medium vectors are indicated by red dots, and the circles in gray (Pará) or black (Rio de Janeiro) are the haplotypes. The diameter of the black and grey circles are proportional to the number of samples sharing a same haplotype (see Table 2) (TIFF 178 kb)
705_2017_3439_MOESM2_ESM.tif (249 kb)
Haplotype network for HPV45 generated by median joining analysis, based on samples of Rio de Janeiro and Pará, medium vectors are indicated by red dots, and the circles in gray (Pará) or black (Rio de Janeiro) are the haplotypes. The diameter of the black and grey circles are proportional to the number of samples sharing a same haplotype (see Table 2) (TIFF 249 kb)
705_2017_3439_MOESM3_ESM.tif (211 kb)
Haplotype network for HPV58 generated by median joining analysis, based on samples of Rio de Janeiro and Pará, medium vectors are indicated by red dots, and the circles in gray (Pará) or black (Rio de Janeiro) are the haplotypes. The diameter of the black and grey circles are proportional to the number of samples sharing a same haplotype (see Table 2) (TIFF 211 kb)
705_2017_3439_MOESM4_ESM.doc (29 kb)
Supplementary material 4 (DOC 29 kb)
705_2017_3439_MOESM5_ESM.xls (110 kb)
Supplementary material 5 (XLS 110 kb)

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Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  • Diogo Lisbôa Basto
    • 1
    • 2
  • João Paulo Vidal
    • 2
  • Valéria Barbosa Pontes
    • 3
  • Shayany Pinto Felix
    • 2
  • Laine Celestino Pinto
    • 3
  • Bruno Moreira Soares
    • 3
  • Luis Felipe Martins
    • 4
  • Flávia Miranda Correa
    • 4
  • Raquel Carvalho Montenegro
    • 5
  • Cláudia Bessa Pereira Chaves
    • 6
  • Liz Maria Almeida
    • 4
  • Miguel Ângelo Martins Moreira
    • 2
    Email author
  1. 1.Genetics DepartmentUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Genetics ProgramInstituto Nacional de CâncerRio de JaneiroBrazil
  3. 3.Department of Woman Health, Faculty of MedicineUniversidade Federal do ParáBelém do ParáBrazil
  4. 4.Population Research DepartmentInstituto Nacional de Câncer (INCA)Rio de JaneiroBrazil
  5. 5.Department of Physiology and PharmacologyUniversidade Federal do CearáFortalezaBrazil
  6. 6.Gynecologic Section and Molecular Carcinogenesis ProgramInstituto Nacional de CâncerRio de JaneiroBrazil

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