Archives of Virology

, Volume 162, Issue 10, pp 2949–2957 | Cite as

Prevalence of S gene mutations within the major hydrophilic region of hepatitis B virus in patients in Dongguan, southern China

  • Siping Li
  • Mingyu Xie
  • Wenrui Li
  • Qi Peng
  • Baimao Zhong
  • Xiaomei LuEmail author
  • Qiang MaEmail author
Original Article


HBsAg point mutations within the major hydrophilic region (MHR) have frequently been reported to be associated with diagnostic failure, vaccine escape and immunotherapy escape. However, the prevalence of escape mutations in chronic hepatitis B (CHB) patients has not been systematically studied in patients from southern China within the past decade. This study aimed to determine the prevalence of escape mutations within the MHR of hepatitis B virus in patients in Dongguan, southern China. Between June 2015 and May 2016, 391 patients who were chronically infected with HBV were enrolled in the study, including 240 patients with the genotype B strain and 151 with the genotype C strain. The most frequent mutated position was s126 (4.3%), followed by s100 (3.3%), s101 (2.8%), s133 (2.8%), s145 (2.3%), s120 (2.0%) and s129 (1.8%). Furthermore, the mutations sY100C, sQ101R/K, sS114A, sP120T, sT/I126A/N/S, sQ129R, sM133L/T/S and sG145R/A were prevalent in at least one genotype, with a frequency higher than 1%, which indicated that these mutations were relatively common. In addition, sQ101K/R was found only in genotype C isolates (P < 0.05), and sT126A was only discovered in genotype B isolates (P = 0.047), indicating that such mutations were genotype-associated mutations. Notably, combinations of escape mutations within the MHR were also frequently discovered in genotypes B (5.0%) and C (6.6%), with no significant difference (P = 0.498). These results indicated that we should increase the surveillance HBsAg mutations among HBV-infected patients in China.



This work was supported by Dongguan Bureau of Science and Technology for the City Key Program of Science and Technology (Project Number: 2013108101038). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Compliance with ethical standards

Ethical standards

This study was approved by the Ethics Committee of Dongguan Eighth People’s Hospital. All participating subjects or their guardians gave signed informed consent before the study.

Conflict of interest

There are no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  1. 1.Dongguan Eighth People’s HospitalDongguanChina
  2. 2.Dongguan Institute of PediatricsDongguanChina
  3. 3.School of Life SciencesZhengzhou UniversityZhengzhouChina

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