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Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars

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Abstract

Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR). A total of 133 symptomatic as well as asymptomatic banana leaf samples from various cultivars were collected from the different regions of India and evaluated for BBTV infection using the RPA assay. BBTV was efficiently detected using crude leaf sap in RPA and the results obtained were consistent with PCR-based detection using purified DNA as template. To our knowledge, this is the first report of reliable diagnosis of BBTV infection by RPA using crude leaf sap as a template.

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References

  1. Banerjee A, Roy S, Behere GT, Roy SS, Dutta SK, Ngachan SV (2014) Identification and characterization of a distinct banana bunchy top virus isolate of Pacific-Indian Oceans group from North-East India. Virus Res 183:41–49

    Article  CAS  PubMed  Google Scholar 

  2. Chen Y, Hu X (2013) High-throughput detection of banana bunchy top virus in banana plants and aphids using real-time TaqMan® PCR. J Virol Methods 193:177–183

    Article  CAS  PubMed  Google Scholar 

  3. Das BK, Jena RC, Samal KC (2009) Optimization of DNA isolation and PCR protocol for RAPD analysis of banana/plantain (Musa spp.). Int J Agric Sci 1(2):21–25

    Article  Google Scholar 

  4. Galal AM (2007) Use of polymerase chain reaction for detecting banana bunchy top nanovirus. Biotechnology 6:53–56

    Article  CAS  Google Scholar 

  5. Hooks CR, Fukuda S, Perez EA, Manandhar R, Wang KH, Wright MG, Almeida RP (2009) Aphid transmission of banana bunchy top virus to bananas after treatment with a bananacide. J Econ Entomol 102:493–499

    Article  PubMed  Google Scholar 

  6. Hu JS, Wang M, Sether D, Xie W, Leonhardt KW (1996) Use of polymerase chain reaction (PCR) to study transmission of banana bunchy top virus by the banana aphid (Pentalonia nigronervosa). Ann App Biol 128:55–64

    Article  CAS  Google Scholar 

  7. Joshi M, Deshpande JD (2011) Polymerase chain reaction: methods, principles and acpplication. Int J Biomed Res 7:81–89

    Google Scholar 

  8. Londono MA, Harmon CL, Polston JE (2016) Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics. Virol J 13:48

    Article  PubMed  PubMed Central  Google Scholar 

  9. Mahadev SR, Thamilarasan SK, Kathithachalam A (2013) PCR detection of banana bunchy top virus (BBTV) at tissue culture level for the production of virus-free planting materials. Int Res J Biol Sci 2(6):22–26

    Google Scholar 

  10. Mansoor S, Qazi J, Amin I, Khatri A, Khan IA, Raza S, Zafar Y, Briddon RW (2005) A PCR-based method, with internal control, for the detection of banana bunchy top virus in banana. Mol Biotechnol 30:167–170

    Article  CAS  PubMed  Google Scholar 

  11. Mekuria TA, Zhang S, Eastwella KC (2014) Rapid and sensitive detection of little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification. J Virol Methods 205:24–30

    Article  CAS  PubMed  Google Scholar 

  12. Peng J, Zhang J, Xia Z, Li Y, Huang J, Fan Z (2012) Rapid and sensitive detection of banana bunchy top virus by loop-mediated isothermal amplification. J Virol Methods 185:254–258

    Article  CAS  PubMed  Google Scholar 

  13. Ponsubrya RK, Alex S, Rajmohan K, Soni KB (2014) Polymerase chain reaction based detection of banana bunchy top virus using coat protein based primers. Int J Agric Environ Biotechnol 7(4):765–767

    Article  Google Scholar 

  14. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, p 2344 (3 volume set)

    Google Scholar 

  15. Selvarajan R, Balasubramanian V, Kavitha K, Kavitha KS, Sathiamoorthy S, Ahlawat YS (2008) Detection of banana bunchy top virus and banana streak Mysore virus by PCR: Impact of storing virus infected banana samples. Indian J Virol 19:28–33

    Google Scholar 

  16. Selvarajan R, Sheeba MM, Balasubramanian V, Rajmohan R, Dhevi NL, Sasireka T (2010) Molecular characterization of geographically different banana bunchy top virus isolates in India. Indian J Virol 21:110–116

    Article  CAS  PubMed  Google Scholar 

  17. Selvarajan R, Balasubramanian V, Dayakar S, Sathiamoorthy S, Ahlawat YS (2010) Evaluation of immunological and molecular techniques for the detection of different isolates of banana bunchy top virus in India. Indian Phytopathol 63(3):333–336

    CAS  Google Scholar 

  18. Selvarajan R, Sheeba MM, Balasubramanian V (2011) Simultaneous detection of episomal banana streak Mysore virus and banana bunchy top virus using multiplex RT-PCR. Curr Sci 100:31–34

    CAS  Google Scholar 

  19. Selvarajan R, Balasubramanian V, Sasireka T (2015) A simple, rapid and solvent free nucleic acid extraction protocol for detection of banana bunchy top virus by polymerase chain reaction and loop-mediated isothermal amplification. Eur J Plant Pathol 142(2):389–396

    Article  CAS  Google Scholar 

  20. Shankar K, Chavan L, Shinde S, Patil B (2011) An improved DNA extraction protocol from four in vitro banana cultivars. Asian J Biotechnol 3:84–90

    Article  CAS  Google Scholar 

  21. Silva G, Bömer M, Nkere C, Kumar PL, Seal SE (2015) Rapid and specific detection of yam mosaic virus by reverse-transcription recombinase polymerase amplification. J Virol Methods 222:138–144

    Article  CAS  PubMed  Google Scholar 

  22. Thomas JE, Dietzgen RG (1991) Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. J Gen Virol 72:217–224

    Article  CAS  PubMed  Google Scholar 

  23. Wu RV, Su HJ (1990) Production of monoclonal antibodies against banana bunchy top virus and their use in enzyme linked immunosorbent assay. J Phytopathol 128:203–208

    Article  CAS  Google Scholar 

  24. Xie WS, Hu JS (1995) Molecular cloning, sequence analysis, and detection of banana bunchy top virus in Hawaii. Phytopathology 85:339–347

    Article  CAS  Google Scholar 

  25. Zhang S, Ravelonandro M, Russell P, McOwen N, Briard P, Bohannon S, Vrient A (2014) Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. J Virol Methods 207:114–120

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by the Department of Biotechnology under the Referral Centre of “National Certification System of Tissue Culture Raised Plants” (NCS-TCP). The authors wish to thank the Head of the Division of Plant Pathology and Director of the Indian Agricultural Research Institute, for providing the necessary lab facilities. We are also grateful to Dr. Akhilesh Mishra of Jain Irrigation Systems Ltd, Jalgaon, and Dr. Vimi Louis, Associate Professor at Kerala Agricultural University, for help with sample collection.

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Authors and Affiliations

  1. Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Reetika Kapoor, Nishant Srivastava, Shailender Kumar, R. K. Saritha, Rakesh Kumar Jain & Virendra Kumar Baranwal

  2. ICAR Research Complex for NEH Region, Manipur Centre, Lamphelpat, Imphal, 795004, India

    Susheel Kumar Sharma

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  1. Reetika Kapoor
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  2. Nishant Srivastava
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  3. Shailender Kumar
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  4. R. K. Saritha
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  5. Susheel Kumar Sharma
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  6. Rakesh Kumar Jain
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  7. Virendra Kumar Baranwal
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Corresponding author

Correspondence to Virendra Kumar Baranwal.

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Funding

This study was funded by the Department of Biotechnology, Government of India, under the National Certification System for Tissue Culture Raised Plants project.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors

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Kapoor, R., Srivastava, N., Kumar, S. et al. Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars. Arch Virol 162, 2791–2796 (2017). https://doi.org/10.1007/s00705-017-3399-9

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  • DOI: https://doi.org/10.1007/s00705-017-3399-9

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