Archives of Virology

, Volume 162, Issue 9, pp 2791–2796 | Cite as

Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars

  • Reetika Kapoor
  • Nishant Srivastava
  • Shailender Kumar
  • R. K. Saritha
  • Susheel Kumar Sharma
  • Rakesh Kumar Jain
  • Virendra Kumar Baranwal
Brief Report

Abstract

Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR). A total of 133 symptomatic as well as asymptomatic banana leaf samples from various cultivars were collected from the different regions of India and evaluated for BBTV infection using the RPA assay. BBTV was efficiently detected using crude leaf sap in RPA and the results obtained were consistent with PCR-based detection using purified DNA as template. To our knowledge, this is the first report of reliable diagnosis of BBTV infection by RPA using crude leaf sap as a template.

Supplementary material

705_2017_3399_MOESM1_ESM.pdf (276 kb)
Supplementary material 1 (PDF 276 kb)

References

  1. 1.
    Banerjee A, Roy S, Behere GT, Roy SS, Dutta SK, Ngachan SV (2014) Identification and characterization of a distinct banana bunchy top virus isolate of Pacific-Indian Oceans group from North-East India. Virus Res 183:41–49CrossRefPubMedGoogle Scholar
  2. 2.
    Chen Y, Hu X (2013) High-throughput detection of banana bunchy top virus in banana plants and aphids using real-time TaqMan® PCR. J Virol Methods 193:177–183CrossRefPubMedGoogle Scholar
  3. 3.
    Das BK, Jena RC, Samal KC (2009) Optimization of DNA isolation and PCR protocol for RAPD analysis of banana/plantain (Musa spp.). Int J Agric Sci 1(2):21–25CrossRefGoogle Scholar
  4. 4.
    Galal AM (2007) Use of polymerase chain reaction for detecting banana bunchy top nanovirus. Biotechnology 6:53–56CrossRefGoogle Scholar
  5. 5.
    Hooks CR, Fukuda S, Perez EA, Manandhar R, Wang KH, Wright MG, Almeida RP (2009) Aphid transmission of banana bunchy top virus to bananas after treatment with a bananacide. J Econ Entomol 102:493–499CrossRefPubMedGoogle Scholar
  6. 6.
    Hu JS, Wang M, Sether D, Xie W, Leonhardt KW (1996) Use of polymerase chain reaction (PCR) to study transmission of banana bunchy top virus by the banana aphid (Pentalonia nigronervosa). Ann App Biol 128:55–64CrossRefGoogle Scholar
  7. 7.
    Joshi M, Deshpande JD (2011) Polymerase chain reaction: methods, principles and acpplication. Int J Biomed Res 7:81–89Google Scholar
  8. 8.
    Londono MA, Harmon CL, Polston JE (2016) Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics. Virol J 13:48CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Mahadev SR, Thamilarasan SK, Kathithachalam A (2013) PCR detection of banana bunchy top virus (BBTV) at tissue culture level for the production of virus-free planting materials. Int Res J Biol Sci 2(6):22–26Google Scholar
  10. 10.
    Mansoor S, Qazi J, Amin I, Khatri A, Khan IA, Raza S, Zafar Y, Briddon RW (2005) A PCR-based method, with internal control, for the detection of banana bunchy top virus in banana. Mol Biotechnol 30:167–170CrossRefPubMedGoogle Scholar
  11. 11.
    Mekuria TA, Zhang S, Eastwella KC (2014) Rapid and sensitive detection of little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification. J Virol Methods 205:24–30CrossRefPubMedGoogle Scholar
  12. 12.
    Peng J, Zhang J, Xia Z, Li Y, Huang J, Fan Z (2012) Rapid and sensitive detection of banana bunchy top virus by loop-mediated isothermal amplification. J Virol Methods 185:254–258CrossRefPubMedGoogle Scholar
  13. 13.
    Ponsubrya RK, Alex S, Rajmohan K, Soni KB (2014) Polymerase chain reaction based detection of banana bunchy top virus using coat protein based primers. Int J Agric Environ Biotechnol 7(4):765–767CrossRefGoogle Scholar
  14. 14.
    Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, p 2344 (3 volume set) Google Scholar
  15. 15.
    Selvarajan R, Balasubramanian V, Kavitha K, Kavitha KS, Sathiamoorthy S, Ahlawat YS (2008) Detection of banana bunchy top virus and banana streak Mysore virus by PCR: Impact of storing virus infected banana samples. Indian J Virol 19:28–33Google Scholar
  16. 16.
    Selvarajan R, Sheeba MM, Balasubramanian V, Rajmohan R, Dhevi NL, Sasireka T (2010) Molecular characterization of geographically different banana bunchy top virus isolates in India. Indian J Virol 21:110–116CrossRefPubMedGoogle Scholar
  17. 17.
    Selvarajan R, Balasubramanian V, Dayakar S, Sathiamoorthy S, Ahlawat YS (2010) Evaluation of immunological and molecular techniques for the detection of different isolates of banana bunchy top virus in India. Indian Phytopathol 63(3):333–336Google Scholar
  18. 18.
    Selvarajan R, Sheeba MM, Balasubramanian V (2011) Simultaneous detection of episomal banana streak Mysore virus and banana bunchy top virus using multiplex RT-PCR. Curr Sci 100:31–34Google Scholar
  19. 19.
    Selvarajan R, Balasubramanian V, Sasireka T (2015) A simple, rapid and solvent free nucleic acid extraction protocol for detection of banana bunchy top virus by polymerase chain reaction and loop-mediated isothermal amplification. Eur J Plant Pathol 142(2):389–396CrossRefGoogle Scholar
  20. 20.
    Shankar K, Chavan L, Shinde S, Patil B (2011) An improved DNA extraction protocol from four in vitro banana cultivars. Asian J Biotechnol 3:84–90CrossRefGoogle Scholar
  21. 21.
    Silva G, Bömer M, Nkere C, Kumar PL, Seal SE (2015) Rapid and specific detection of yam mosaic virus by reverse-transcription recombinase polymerase amplification. J Virol Methods 222:138–144CrossRefPubMedGoogle Scholar
  22. 22.
    Thomas JE, Dietzgen RG (1991) Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. J Gen Virol 72:217–224CrossRefPubMedGoogle Scholar
  23. 23.
    Wu RV, Su HJ (1990) Production of monoclonal antibodies against banana bunchy top virus and their use in enzyme linked immunosorbent assay. J Phytopathol 128:203–208CrossRefGoogle Scholar
  24. 24.
    Xie WS, Hu JS (1995) Molecular cloning, sequence analysis, and detection of banana bunchy top virus in Hawaii. Phytopathology 85:339–347CrossRefGoogle Scholar
  25. 25.
    Zhang S, Ravelonandro M, Russell P, McOwen N, Briard P, Bohannon S, Vrient A (2014) Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. J Virol Methods 207:114–120CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Reetika Kapoor
    • 1
  • Nishant Srivastava
    • 1
  • Shailender Kumar
    • 1
  • R. K. Saritha
    • 1
  • Susheel Kumar Sharma
    • 2
  • Rakesh Kumar Jain
    • 1
  • Virendra Kumar Baranwal
    • 1
  1. 1.Division of Plant Pathology, Advanced Centre for Plant VirologyIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.ICAR Research Complex for NEH RegionManipur CentreImphalIndia

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