Archives of Virology

, Volume 162, Issue 8, pp 2297–2303 | Cite as

Application of gold nanoparticle-assisted PCR for equine herpesvirus 1 diagnosis in field samples

  • Dalia M. El-Husseini
  • Nashwa M. Helmy
  • Reham H. Tammam
Original Article
First Online:
Received:
Accepted:

Abstract

Equine herpesvirus 1 (EHV-1) is one of the most significant pathogens that affects equine species worldwide, causing sporadic abortion, neonatal deaths, chorioretinopathy, as well as neurological and upper respiratory tract diseases. Currently, conventional PCR targeting different genes is used widely for the molecular detection of EHV-1, but the low viral titer in some clinical samples can lead to false negative results. In this study, we aimed to assess gold nanoparticle (GNP)-assisted PCR as an inexpensive, highly efficient, and sensitive method for the detection of EHV-1, and to compare its results with conventional PCR and real-time quantitative PCR (qPCR). Out of 83 field samples, 28.9%, 26.5%, and 15.6% were EHV-1-positive by qPCR, GNP-assisted PCR and conventional PCR, respectively. All three techniques specifically target the viral glycoprotein B gene. The optimized GNP-assisted PCR showed no cross-reactivity with EHV-1-negative samples (diagnosed by qPCR). GNP-assisted PCR is a powerful new tool for EHV-1 detection and surveillance, because of its simplicity, sensitivity and specificity. It can be used as an alternative to qPCR in laboratories that cannot afford the expense of a qPCR system.

Keywords

Polymerase Chain Reaction Porcine Epidemic Diarrhea Virus Conventional Polymerase Chain Reaction Equine Herpesvirus Mink Enteritis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

We thank Dr James Allen of AuthorAID for assistance with manuscript preparation and language use.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

No funding was received for this study.

Supplementary material

705_2017_3379_MOESM1_ESM.doc (1.1 mb)
Supplementary material 1 (DOC 1168 kb)

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Biotechnology DepartmentAnimal Health Research InstituteGizaEgypt
  2. 2.Chemistry Department, Faculty of ScienceCairo UniversityGizaEgypt

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