Archives of Virology

, Volume 162, Issue 8, pp 2409–2413 | Cite as

A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines

  • Ryosuke Matsuura
  • Marina Takada
  • Takehiro Kokuho
  • Takamitsu Tsuboi
  • Ken-ichiro Kameyama
  • Kaoru Takeuchi
Brief Report


The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (FL288I-EGFP). FL288I-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.


Bovine parainfluenza virus type 3 Fusion protein Cell-specific adaptation 



This work was supported in part by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (No. AGD28067) and from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 25460561). We thank T. Ohkura (Northwestern University) for critical reading.

Compliance with ethical standards

Conflict of interest

The authors have declared that no conflict of interest exists.


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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Laboratory of Environmental Microbiology, Faculty of MedicineUniversity of TsukubaTsukubaJapan
  2. 2.Biologics Production, Center for Animal Disease Control and PreventionNational Institute of Animal Health, NAROTsukubaJapan
  3. 3.Viral Diseases and Epidemiology Research DivisionNational Institute of Animal Health, NAROTsukubaJapan

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