A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines

Archives of Virology volume 162, pages2409–2413(2017)Cite this article

Abstract

The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (FL288I-EGFP). FL288I-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.

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Acknowledgements

This work was supported in part by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (No. AGD28067) and from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 25460561). We thank T. Ohkura (Northwestern University) for critical reading.

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Affiliations

  1. Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan

    Ryosuke Matsuura, Marina Takada & Kaoru Takeuchi

  2. Biologics Production, Center for Animal Disease Control and Prevention, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, 305-0856, Japan

    Takehiro Kokuho

  3. Viral Diseases and Epidemiology Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, 305-0856, Japan

    Takamitsu Tsuboi & Ken-ichiro Kameyama

Authors
  1. Ryosuke Matsuura
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  2. Marina Takada
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  3. Takehiro Kokuho
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  4. Takamitsu Tsuboi
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  5. Ken-ichiro Kameyama
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  6. Kaoru Takeuchi
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Corresponding author

Correspondence to Kaoru Takeuchi.

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Matsuura, R., Takada, M., Kokuho, T. et al. A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines. Arch Virol 162, 2409–2413 (2017). https://doi.org/10.1007/s00705-017-3378-1

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Keywords

  • Bovine parainfluenza virus type 3
  • Fusion protein
  • Cell-specific adaptation