A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines
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The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (FL288I-EGFP). FL288I-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.
KeywordsBovine parainfluenza virus type 3 Fusion protein Cell-specific adaptation
This work was supported in part by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (No. AGD28067) and from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 25460561). We thank T. Ohkura (Northwestern University) for critical reading.
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Conflict of interest
The authors have declared that no conflict of interest exists.
- 3.Karron RA, Collins PL (2013) Parainfluenza viruses. In: Knipe DM, Howley PM (eds) Fields virology, 6th edn. Lippincott/The Williams & Wilkins Co, Philadelphia, pp 996–1023Google Scholar
- 4.Ohkura T, Kokuho T, Konishi M, Kameyama K, Takeuchi K (2013) Complete genome sequences of bovine parainfluenza virus type 3 strain BN-1 and vaccine strain BN-CE. Genome A e00247-12Google Scholar