Development of a real-time recombinase polymerase amplification assay for rapid and sensitive detection of porcine circovirus 2

  • Jianchang Wang
  • Jinfeng Wang
  • Libing Liu
  • Wanzhe Yuan
Original Article

DOI: 10.1007/s00705-017-3368-3

Cite this article as:
Wang, J., Wang, J., Liu, L. et al. Arch Virol (2017). doi:10.1007/s00705-017-3368-3
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Abstract

Porcine diseases associated with porcine circovirus 2 (PCV-2) infection have resulted in significant economic losses worldwide. A real-time recombinase polymerase amplification (RPA) assay was developed to detect PCV-2 using primers and an exo probe specific for the ORF2 gene. The reaction process can be completed in 20 min at 38 °C. The assay only detects PCV-2, as there was no cross-reaction with other pathogens important in pigs. Using the PCV-2 genomic DNA as template, the analytical sensitivity of the real-time RPA was 103 copies. The assay performance was evaluated by testing 38 field samples and compared with real-time PCR. The two assays demonstrated a 100% diagnostic agreement, and PCV-2 DNA was detected in 26 samples. The R2 value of real-time RPA and real-time PCR was 0.954 by linear regression analysis. The real-time RPA assay provides an alternative tool for rapid, simple, and reliable detection of PCV-2, especially in remote and rural areas.

Funding information

Funder NameGrant NumberFunding Note
Science and Technology Project Foundation of Hebei Province
  • 16226604D

Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Jianchang Wang
    • 1
  • Jinfeng Wang
    • 1
  • Libing Liu
    • 1
  • Wanzhe Yuan
    • 2
  1. 1.Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine BureauShijiazhuangChina
  2. 2.College of Veterinary MedicineAgricultural University of HebeiBaodingChina

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