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Archives of Virology

, Volume 162, Issue 8, pp 2437–2440 | Cite as

Complete genomic characterization of milk vetch dwarf virus isolates from cowpea and broad bean in Anhui province, China

  • Chenhua Zhang
  • Hongying Zheng
  • Dankan Yan
  • Kelei Han
  • Xijiao Song
  • Yong Liu
  • Dongfang ZhangEmail author
  • Jianping ChenEmail author
  • Fei YanEmail author
Annotated Sequence Record

Abstract

Cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in Hefei city, Anhui province, China, in 2014. Symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (MDV) by PCR. The complete genomes of MDV isolates from cowpea and broad bean were sequenced. Each of them had eight genomic DNAs that differed between the two isolates by 10.7% in their overall nucleotide sequences. In addition, the MDV genomes from cowpea and broad bean were associated with two and three alphasatellite DNAs, respectively. This is the first report of MDV on cowpea in China and the first complete genome sequences of Chinese MDV isolates.

Keywords

Complete Genome Sequence Broad Bean Vigna Unguiculata Severe Stunting Reassortment Event 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Prof. M. J. Adams, Stevenage, Herts, UK, for correcting the English in the manuscript.

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors and is in compliance with ethical standards for research.

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was funded by Special Fund for Agro-Scientific Research in the Public Interest (grant number: 201303028) and the International Science & Technology Cooperation Program of China (grant number: 2015DFA30700).

Supplementary material

705_2017_3348_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 49 kb)
705_2017_3348_MOESM2_ESM.doc (52 kb)
Supplementary material 2 (DOC 52 kb)
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Supplementary material 3 (DOC 43 kb)
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Supplementary material 4 (DOC 95 kb)
705_2017_3348_MOESM5_ESM.tif (339 kb)
Suppl. Fig. S1: The different C-ends of the U2 protein among MDV isolates (TIFF 339 kb)
705_2017_3348_MOESM6_ESM.tif (471 kb)
Suppl. Fig. S2: The different N-termini of the U4 protein among MDV isolates (TIFF 471 kb)

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.College of Life SciencesFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.State Key Laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhouChina
  3. 3.Institute of Plant Protection and Agricultural Product Quality and SafetyAnhui Academy of Agricultural SciencesHefeiChina
  4. 4.Institute of Plant ProtectionHunan Academy of Agricultural SciencesChangshaChina

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