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Archives of Virology

, Volume 162, Issue 7, pp 2083–2090 | Cite as

Incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in Thailand

  • S. Cheewachaiwit
  • N. Warin
  • B. Phuangrat
  • S. Rukpratanporn
  • O. Gajanandana
  • C. H. Balatero
  • O. Chatchawankanphanich
Brief Report

Abstract

Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3’ RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.

Keywords

Cucurbit aphid-borne yellows virus Melon aphid-borne yellows virus Suakwa aphid-borne yellows virus Luteoviridae Polerovirus Recombination 

Notes

Acknowledgements

This research was supported by Cluster and Program Management Office (CPMO), National Science and Technology Development Agency (NSTDA) and the Hortigenetics Research, (S.E. Asia) Ltd., East-West Seeds. Miss S. Cheewachaiwit was financially supported for her study in Master degree by NSTDA-University-Industry Research Collaboration (NUI-RC), NSTDA.

Compliance with ethical standards

This project was a collaborative research program between the NSTDA, a public research agency, and Hortigenetics Research, (S.E. Asia) Ltd., East-West Seeds, a commercial company. Funding was provided by both parties and was managed through the CPMO, NSTDA. In addition, Miss Sirirat Cheewachaiwit, a researcher from the company, who pursued her master degree at the Center for Agricultural Biotechnology, Kasetsart University under the supervision of Dr. Orawan Chatchawankanphanich, also received the scholarship from the NSTDA under the NSTDA-NUI-RC program. Dr. Conrado H. Balatero, Miss Sirirat Cheewachaiwit and Dr. Orawan Chatchawankanphanich declared that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 1 (DOC 80 kb)
705_2017_3332_MOESM2_ESM.doc (73 kb)
Supplementary material 2 (DOC 73 kb)
705_2017_3332_MOESM3_ESM.doc (84 kb)
Supplementary material 3 (DOC 84 kb)

References

  1. 1.
    Abou-Jawdah Y, Sobh H, Fayyad A (1997) First report of Cucurbit aphid-borne yellows luteovirus in Lebanon. Plant Dis 81:1331CrossRefGoogle Scholar
  2. 2.
    Bananej K, Desbiez C, Wipf-Scheibel C, Vahdat I, Khey-Pour A, Ahoonmanesh A, Lecoq H (2006) First report of Cucurbit aphid-borne yellows virus in Iran causing yellows on four cucurbit crops. Plant Dis 90:526CrossRefGoogle Scholar
  3. 3.
    Boubourakas IN, Avgelis AD, Kyriakopulou PE, Katis NI (2006) Occurrence of yellowing viruses (Beet Pseudo-yellow virus, Cucurbit yellow stunting disorder virus and Cucurbit aphid-borne yellows virus) affecting cucurbits in Greece. Plant Pathol 55:276–283CrossRefGoogle Scholar
  4. 4.
    Chiemsombat P, Gajanadana O, Warin N, Hongprayoon R, Bhunchoth A, Pongsapich P (2008) Biological and molecular characterization of tospoviruses in Thailand. Arch Virol 153:571–577CrossRefPubMedGoogle Scholar
  5. 5.
    Ito T, Samretwanich K, Sharma P, Ikegami M (2008) Yellow leaf curl disease of pumpkin in Thailand associated with Squash leaf curl China virus. Plant Pathol 57:766–772CrossRefGoogle Scholar
  6. 6.
    Juarez M, Truniger V, Aranda MA (2004) First report of Cucurbit aphid-borne yellows virus in Spain. Plant Dis 88:907CrossRefGoogle Scholar
  7. 7.
    Knierim D, Deng TC, Tsai WS, Green SK, Kenyon L (2010) Molecular identification of three distinct polerovirus species and a recombinant Cucurbit aphid borne yellows virus strain infecting cucurbit crops in Taiwan. Plant Pathol 59:991–1002CrossRefGoogle Scholar
  8. 8.
    Knierim D, Tsai WS, Deng TC, Green SK, Kenyon L (2013) Full-length genome sequences of four polerovirus isolates infecting cucurbits in Taiwan determined from total RNA extracted from field samples. Plant Pathol 62:633–641CrossRefGoogle Scholar
  9. 9.
    Knierim D, Tsai WS, Maiss E, Kenyon L (2014) Molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. Arch Virol 159:1459–1465CrossRefPubMedGoogle Scholar
  10. 10.
    Lecoq H, Bourdin D, Wipf-Scheibel C, Bon M, Lot H, Lemaire O, Herrbach E (1992) A new yellowing disease of cucurbits caused by a luteovirus, cucurbit aphid-borne yellows virus. Plant Pathol 41:749–761CrossRefGoogle Scholar
  11. 11.
    Lemaire OJ, Gubler WD, Valencia J, Lecoq H, Falk BW (1993) First report of cucurbit aphid-borne yellows luteovirus in the United States. Plant Dis 77:1169CrossRefGoogle Scholar
  12. 12.
    Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length human immunodeficiency virus type 1 genome from subtype C- infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160PubMedPubMedCentralGoogle Scholar
  13. 13.
    Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1:1–5CrossRefGoogle Scholar
  14. 14.
    Mnari-Hattab M, Kummert J, Roussel S, Ezzaier K, Zouba A, Jijakli MH (2005) First report of Cucurbit aphid-borne yellows virus in Tunisia causing yellows on five cucurbitacious species. Plant Dis 89:776CrossRefGoogle Scholar
  15. 15.
    Noda S, Kittipakorn K, Inchan P, Wanapee L, Deema N (1993) Distribution of cucurbit viruses and reactions of some cucurbit species to certain viruses. In: Proceedings of the 31st Kasetsart University annual conference: plants, Bangkok, pp 341–347Google Scholar
  16. 16.
    Omar AF, Bagdady NA (2012) Cucurbit aphid-borne yellows virus in Egypt. Phytoparasitica 40:177–184CrossRefGoogle Scholar
  17. 17.
    Papayiannis LC, Ioannou N, Boubourakas IN, Dovas CI, Katis NI, Falk BW (2005) Incidence of viruses infecting cucurbits in Cyprus. J Phytopathol 153:530–535CrossRefGoogle Scholar
  18. 18.
    Relevante CA, Cheewachaiwit S, Hassani-Mehraban A, Peters D (2012) Molecular characterization of Cucurbit aphid-borne yellows virus isolates associated with “Namamarakao” in the Philippines and “Mara Ba” in Thailand. In: The international conference on tropical and sub-tropical plant disease, 7–10 February 2012. The Empress Hotel, Chiang Mai, p 87Google Scholar
  19. 19.
    Shang QX, Xiang HY, Han CG, Li DW, Yu JL (2009) Distribution and molecular diversity of three cucurbit-infecting poleroviruses in China. Virus Res 145:341–346CrossRefPubMedGoogle Scholar
  20. 20.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Tomassoli L, Meneghini M (2007) First report of Cucurbit aphid-borne yellows virus in Italy. Plant Pathol 56:720CrossRefGoogle Scholar
  22. 22.
    Verwoerd TC, Dekker BM, Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res 17:2362CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Vucurovic A, Bulajic A, Stankovic I, Ristic D, Berenji J, Jovic’ J, Krstic’ B (2011) First report of the occurrence of Cucurbit aphid-borne yellows virus on oilseed pumpkin in Serbia. Plant Dis 95:1035CrossRefGoogle Scholar
  24. 24.
    Xiang HY, Shang QX, Han CG, Li DW, Yu JL (2007) First report on the occurrence of Cucurbit aphid-borne yellows virus on nine cucurbitaceous species in China. New Dis Rep 15:9Google Scholar
  25. 25.
    Xiang HY, Shang QX, Han CG, Li DW, Yu JL (2008) Complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in China. Arch Virol 153:1155–1160CrossRefPubMedGoogle Scholar
  26. 26.
    Yardimci N, Özgönen H (2007) First report of Cucurbit aphid-borne yellows virus in Turkey. Aust Plant Dis Notes 2:59CrossRefGoogle Scholar
  27. 27.
    Zitter TA, Banik MT (1984) Virus diseases of cucurbits cooperative extension; fact sheet. Cornell University Plant Pathology Department, New York. http://vegetablemdonline.ppath.cornell.edu/factsheets/Viruses_Cucurbits.htm. Accessed 30 Jan 2017

Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Center for Agricultural BiotechnologyKasetsart UniversityNakhon PathomThailand
  2. 2.Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)BangkokThailand
  3. 3.Plant Pathology DepartmentHortigenetics Research (S.E. Asia) Limited East-West SeedsChiang MaiThailand
  4. 4.National Center for Genetic Engineering and BiotechnologyNational Science and Technology Development AgencyPathum ThaniThailand

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