Archives of Virology

, Volume 162, Issue 7, pp 2083–2090 | Cite as

Incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in Thailand

  • S. Cheewachaiwit
  • N. Warin
  • B. Phuangrat
  • S. Rukpratanporn
  • O. Gajanandana
  • C. H. Balatero
  • O. Chatchawankanphanich
Brief Report


Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3’ RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.


Cucurbit aphid-borne yellows virus Melon aphid-borne yellows virus Suakwa aphid-borne yellows virus Luteoviridae Polerovirus Recombination 



This research was supported by Cluster and Program Management Office (CPMO), National Science and Technology Development Agency (NSTDA) and the Hortigenetics Research, (S.E. Asia) Ltd., East-West Seeds. Miss S. Cheewachaiwit was financially supported for her study in Master degree by NSTDA-University-Industry Research Collaboration (NUI-RC), NSTDA.

Compliance with ethical standards

This project was a collaborative research program between the NSTDA, a public research agency, and Hortigenetics Research, (S.E. Asia) Ltd., East-West Seeds, a commercial company. Funding was provided by both parties and was managed through the CPMO, NSTDA. In addition, Miss Sirirat Cheewachaiwit, a researcher from the company, who pursued her master degree at the Center for Agricultural Biotechnology, Kasetsart University under the supervision of Dr. Orawan Chatchawankanphanich, also received the scholarship from the NSTDA under the NSTDA-NUI-RC program. Dr. Conrado H. Balatero, Miss Sirirat Cheewachaiwit and Dr. Orawan Chatchawankanphanich declared that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 1 (DOC 80 kb)
705_2017_3332_MOESM2_ESM.doc (73 kb)
Supplementary material 2 (DOC 73 kb)
705_2017_3332_MOESM3_ESM.doc (84 kb)
Supplementary material 3 (DOC 84 kb)


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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Center for Agricultural BiotechnologyKasetsart UniversityNakhon PathomThailand
  2. 2.Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)BangkokThailand
  3. 3.Plant Pathology DepartmentHortigenetics Research (S.E. Asia) Limited East-West SeedsChiang MaiThailand
  4. 4.National Center for Genetic Engineering and BiotechnologyNational Science and Technology Development AgencyPathum ThaniThailand

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