Complete genome sequences of cowpea polerovirus 1 and cowpea polerovirus 2 infecting cowpea plants in Burkina Faso
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The full-length genome sequences of two novel poleroviruses found infecting cowpea plants, cowpea polerovirus 1 (CPPV1) and cowpea polerovirus 2 (CPPV2), were determined using overlapping RT-PCR and RACE-PCR. Whereas the 5845-nt CPPV1 genome was most similar to chickpea chlorotic stunt virus (73% identity), the 5945-nt CPPV2 genome was most similar to phasey bean mild yellow virus (86% identity). The CPPV1 and CPPV2 genomes both have a typical polerovirus genome organization. Phylogenetic analysis of the inferred P1-P2 and P3 amino acid sequences confirmed that CPPV1 and CPPV2 are indeed poleroviruses. Four apparently unique recombination events were detected within a dataset of 12 full polerovirus genome sequences, including two events in the CPPV2 genome. Based on the current species demarcation criteria for the family Luteoviridae, we tentatively propose that CPPV1 and CPPV2 should be considered members of novel polerovirus species.
KeywordsRibosomal Frameshift Species Demarcation Criterion Beckman Coulter Genomic Accession Number NC008249 Unique Recombination Event
EP’s PhD fellowship was funded by the French Embassy of Togo (N°: 808087J; 836014F; 864988K) and International Foundation for Science (N°: C/5489-1). PR is supported by EU grant FP7-PEOPLE-2013-IOF (N° PIOF-GA-2013-622571). We acknowledge Ecole Superieur d’Agronomie of the University of Lomé, which accepted to shelter EP’s IFS funds, and the Laboratoire Mixte International Patho-Bios, which supported sample collection.
Compliance with ethical standards
This study was funded by the French Embassy of Togo (N°: 808087J; 836014F; 864988K.), International Foundation for Science (N°: C/5489-1) and EU grant FP7-PEOPLE-2013-IOF (N° PIOF-GA-2013-622571).
Conflict of interest
Author EP has received research grants from the French Embassy of Togo (N°: 808087J; 836014F; 864988K) and the International Foundation for Science (N°: C/5489-1). Author PR has received an EU grant FP7-PEOPLE-2013-IOF (N° PIOF-GA-2013-622571). Author EP and PR declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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