Archives of Virology

, Volume 162, Issue 7, pp 2053–2059 | Cite as

Cloning, sequencing and characterization of the genome of a recombinant norovirus of the rare genotype GII.P7/GII.6 in China

  • Hui Cai
  • Yongxin Yu
  • Miao Jin
  • Yingjie Pan
  • Shuling Yan
  • Yongjie WangEmail author
Brief Report


The genome sequence of a rare recombinant norovirus (NoV) genotype obtained from clinical samples in China was determined using one-step reverse transcription PCR. It was identified as the GII.P7/GII.6 genotype using both phylogenetic and SimPlot analyses. A high degree of variability was observed in the P2 subdomain, especially in the B-loop structure. The recombination breakpoints of all available GII.P7/GII.6 strains were mapped to two different positions within the RdRp region, both of which were at least 40 nt upstream of the overlap of ORF1 and 2. The GII.P7/GII.6 genotype appears to have been circulating in Asia for at least 10 years.


Capsid Protein Recombinant Strain Recombination Breakpoint Amino Acid Multiple Sequence Alignment SimPlot Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported partially by the National Natural Science Foundation of China (41376135, 31570112 and 31601570) and the Special Fund for the Development of Science and Technology of Shanghai Ocean University (2015).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

705_2017_3325_MOESM1_ESM.pdf (162 kb)
Supplementary material 1 (PDF 161 kb)


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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Hui Cai
    • 1
  • Yongxin Yu
    • 1
    • 2
  • Miao Jin
    • 3
  • Yingjie Pan
    • 1
    • 2
  • Shuling Yan
    • 1
    • 4
  • Yongjie Wang
    • 1
    • 2
    Email author
  1. 1.College of Food Science and TechnologyShanghai Ocean UniversityShanghaiChina
  2. 2.Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai)Ministry of AgricultureShanghaiChina
  3. 3.National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina
  4. 4.Institute of Biochemistry and Molecular Cell BiologyUniversity of GöttingenGöttingenGermany

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