Genetic characterization of Cacipacoré virus from ticks collected in São Paulo State, Brazil
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Cacipacoré virus (CPCV) is a potential emerging virus classified in the genus Flavivirus, family Flaviviridae. In the present study, we present the genetic characterization of a CPCV isolated from ticks (Amblyomma cajennense) collected from a sick capybara (Hydrochoerus hydrochaeris) in São Paulo State, Brazil. The CPCV isolate shares the typical genomic organization of flaviviruses with 10,857 nucleotides in length and a single open reading frame of 10,284 nucleotides encoding a polyprotein of 3,427 amino acids. Phylogenetic analysis revealed that CPCV is unique, as a potentially tick-borne virus, in the Japanese encephalitis virus serogroup.
KeywordsJapanese Encephalitis Virus Amazon Region Japanese Encephalitis Virus Complete Amino Acid Sequence Single Open Reading Frame
The authors are grateful to Adriana Moreira Soares, Vanessa Danielle Menjon Muller, Flávio Lauretti and Carol Kobori da Fonseca for their technical assistance. We also give thanks to Maurício Lacerda Nogueira, Benedito Antônio Lopes da Fonseca, Gabor Pali and Jorge Eduardo Moreira for critical review of the final version of this manuscript.
Compliance with ethical standards
This work was supported by Sao Paulo Research Foundation (FAPESP), Grants no. 2009/ 08932-4 and 2012/08778-8 and Scholarships No. 12/24150-9; 16/01414-1.
Conflict of interest
The authors declare that they have no competing interests.
This study was approved by the Ethics Committee on Animal Experimentation of the Medical School of Ribeirao Preto, University of São Paulo (166/2009).
- 1.Batista PM, Andreotti R, Almeida PS, Marques AC, Rodrigues SG, Chiang JO, Vasconcelos PF (2013) Detection of arboviruses of public health interest in free-living New World primates (Sapajus spp.; Alouatta caraya) captured in Mato Grosso do Sul, Brazil. Revista da Sociedade Brasileira de Medicina Tropical 46:684–690CrossRefPubMedGoogle Scholar
- 13.Moureau G, Cook S, Lemey P, Nougairede A, Forrester NL, Khasnatinov M, Charrel RN, Firth AE, Gould EA, de Lamballerie X (2015) New insights into flavivirus evolution, taxonomy and biogeographic history, extended by analysis of canonical and alternative coding sequences. PLoS One 10:e0117849CrossRefPubMedPubMedCentralGoogle Scholar