Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028
- 502 Downloads
Molecular characterization of the most common dsRNA element from Trichoderma atroviride indicated that it comprised 8,566 bp and encoded two large open reading frames (ORF1 and 2). The two ORFs were found to overlap by 46 bp with a typical (−1) slippery heptanucelotide sequence. The deduced protein sequences of ORF1 and ORF2 showed significant similarities to those of known mycoviral structural proteins and RNA-dependent RNA polymerases, respectively. Phylogenetic analysis indicated that this dsRNA is a member of a distinct species related to a group of unclassified mycoviruses; therefore, it was named Trichoderma atroviride mycovirus 1 (TaMV1).
KeywordsTrichoderma atroviride Mycovirus Lentinula edodes Shiitake
This work was supported by the Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and in part by the NRF grant by MSIP (2015R1A2A1A10055684). We thank the Institute of Molecular Biology and Genetics at Chonbuk National University for kindly providing the facilities for this research. D-H Kim was supported by “Research Base Construction Fund Support Program” funded by Chonbuk National University in 2016.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any experiments involving human participants or animals.
- 1.Tokimoto K (1985) Physiological studies on antagonism between Lentinula edodes and Trichoderma spp. in bedlogs of the former (in Japanese). Rep Tottori Mycol Inst 23:1–54Google Scholar
- 3.Seaby D (1998) Trichoderma as a weed mould or pathogen in mushroom cultivation. In: Harmann GE, Kubicek CP (eds) Trichoderma and gliocladium, vol 2. Taylor and Francis Ltd, London, pp 267–288Google Scholar
- 4.Kredics L, Garcia Jimenez L, Naeimi S, Czifra D, Urban P, Manczinger L, Vagvolgyi C, Hatvani L (2010) A challenge to mushroom growers: the green mould disease of cultivated champignons. In: Mendez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology, vol 1. Formatex Research Center, Badajoz, pp 295–305Google Scholar
- 5.Van Alfen NK (1986) Hypovirulence of Endothia (Cryphonectria) parasitica and Rhizoctonia solani. In: Buck KW (ed) Fungal virology. CRC Press, Boca Raton, pp 143–162Google Scholar
- 10.Wang M, Wang Y, Sun X, Cheng J, Fu Y, Liu H, Jiang D, Ghabrial SA, Xie J (2015) Characterization of a novel megabirnavirus from Sclerotinia sclerotiorum reveals horizontal gene transfer from single-stranded RNA virus to double-stranded RNA virus. J Virol 89:8567–8579CrossRefPubMedPubMedCentralGoogle Scholar
- 21.Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New YorkGoogle Scholar
- 24.Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman, San FranciscoGoogle Scholar