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Archives of Virology

, Volume 162, Issue 3, pp 891–895 | Cite as

Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae

  • Adriana Illana
  • Marco Marconi
  • Julio Rodríguez-Romero
  • Ping Xu
  • Tamas Dalmay
  • Mark D. Wilkinson
  • Maria Ángeles Ayllón
  • Ane Sesma
Annotated Sequence Record

Abstract

In this study we characterize a novel positive and single stranded RNA (ssRNA) mycovirus isolated from the rice field isolate of Magnaporthe oryzae Guy11. The ssRNA contains a single open reading frame (ORF) of 2,373 nucleotides in length and encodes an RNA-dependent RNA polymerase (RdRp) closely related to ourmiaviruses (plant viruses) and ourmia-like mycoviruses. Accordingly, we name this virus Magnaporthe oryzae ourmia-like virus 1 (MOLV1). Although phylogenetic analysis suggests that MOLV1 is closely related to ourmia and ourmia-like viruses, it has some features never reported before within the Ourmiavirus genus. 3’ RLM-RACE (RNA ligase-mediated rapid amplification of cDNA ends) and extension poly(A) tests (ePAT) suggest that the MOLV1 genome contains a poly(A) tail whereas the three cytosine and the three guanine residues present in 5’ and 3’ untranslated regions (UTRs) of ourmia viruses are not observed in the MOLV1 sequence. The discovery of this novel viral genome supports the hypothesis that plant pathogenic fungi may have acquired this type of viruses from their host plants.

Keywords

Plant Virus Rice Blast Fungus Guanine Residue Fungal Host ssRNA Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

A.S. is funded by the Spanish Research council (grant ref. BIO2014-53211-R), the EC (REA grant agreement n. 304039) and the Community of Madrid (grant ref. S2013/ABI-2734).

Compliance with ethical standards and conflict of interest

This paper is in compliance with ethical standards for research. The authors declare no conflicts of interest.

Supplementary material

705_2016_3144_MOESM1_ESM.pdf (768 kb)
Supplementary material 1 (PDF 768 kb)

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Centro de Biotecnología y Genómica de PlantasUniversidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM)MadridSpain
  2. 2.Departamento de Biotecnología-Biología VegetalEscuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPMMadridSpain
  3. 3.School of Biological SciencesUniversity of East Anglia, Norwich Research ParkNorwichUK

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