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Archives of Virology

, Volume 162, Issue 3, pp 713–720 | Cite as

Development of a fluorescence resonance energy transfer–based intracellular assay to identify novel enterovirus 71 antivirals

  • Wen-Wen Lu
  • Fang-Yen Kung
  • Po-An Deng
  • Yu-Chu Lin
  • Cheng-Wen Lin
  • Szu-Hao Kung
Original Article
  • 180 Downloads

Abstract

Enterovirus 71 (EV71) is considered one of the most virulent pathogens in the family Picornaviridae. However, there have been no effective treatments for the severe complications caused by EV71. Development of new drugs against targets that are essential for viral replication often requires screening large collections of compounds, for which a high-throughput screening platform is needed. In this study, a drug-screening platform was developed based on a genetically engineered cell line that displays fluorescence resonance energy transfer (FRET) and shows a real-time and quantifiable impairment of FRET upon EV71 infection. A library of small molecules consisting of 1280 compounds with defined bioactivities was used for screening drugs with anti-EV71 activity; accurate, rapid, and robust results were obtained from this screening procedure. Ten drugs were identified in the primary screening, and their antiviral activities were indicated by dose-dependent elevation of FRET. Among these, AC-93253, mitoxantrone and N-bromoacetamide had not been reported as enterovirus inhibitors, and it was confirmed that they were able to suppress viral yields in a dose-dependent manner. Taken together, these studies demonstrate the feasibility of this FRET-based platform for efficient screening and identification of novel compounds with activity against EV71 infection.

Keywords

Ouabain Mitoxantrone Fluorescence Resonance Energy Transfer Internal Ribosome Entry Site EV71 Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was funded by the Ministry of Education of Taiwan (a grant from Ministry of Education, Aim for the Top University Plan [Grant 104AC-D104]) and the Cheng Hsin General Hospital Foundation (Grant 102-61).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

705_2016_3143_MOESM1_ESM.pdf (40 kb)
Supplementary material 1 (PDF 40 kb)

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Department of Clinical PathologyCheng Hsin General HospitalTaipeiTaiwan, R.O.C.
  2. 2.Department of Biotechnology and Laboratory Science in MedicineNational Yang-Ming UniversityTaipeiTaiwan, R.O.C.
  3. 3.Department of Medical Laboratory Science and BiotechnologyChina Medical UniversityTaichungTaiwan, R.O.C.

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