High throughput sequencing reveals a novel fabavirus infecting sweet cherry
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The genus Fabavirus currently consists of five species represented by viruses that infect a wide range of hosts but none reported from temperate climate fruit trees. A virus with genomic features resembling fabaviruses (tentatively named Prunus virus F, PrVF) was revealed by high throughput sequencing of extracts from a sweet cherry tree (Prunus avium). PrVF was subsequently shown to be graft transmissible and further identified in three other non-symptomatic Prunus spp. from different geographical locations. Two genetic variants of RNA1 and RNA2 coexisted in the same samples. RNA1 consisted of 6,165 and 6,163 nucleotides, and RNA2 consisted of 3,622 and 3,468 nucleotides.
KeywordsHigh Throughput Sequencing Sweet Cherry Sweet Cherry Cultivar Prune Dwarf Virus Cherry Rootstock
This work is partially supported by USDA NIFA HATCH Project No. WNP00754 to the Agriculture Research Center, Washington State University, Pullman, WA, 99164-6240, USA. All plants propagated in this study were maintained in a United States Department of Agriculture Animal and Plant Health Inspection Service (USDA-APHIS) compliant growth room under Permit Number P526P-14-03889.
Compliance with ethical standards
Conflict of interest
All authors declare no conflict of interest.
This research does not contain studies with human or animal participants performed by any of the authors.
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