The genomic sequences of five Japanese isolates of citrus vein enation virus (CVEV) isolates that induce vein enation were determined and compared with that of the Spanish isolate VE-1. The nucleotide sequences of all Japanese isolates were 5,983 nt in length. The genomic RNA of Japanese isolates had five potential open reading frames (ORF 0, ORF 1, ORF 2, ORF 3, and ORF 5) in the positive-sense strand. The nucleotide sequence identity among the Japanese isolates and Spanish isolate VE-1 ranged from 98.0% to 99.8%. Comparison of the partial amino acid sequences of ten Japanese isolates and three Spanish isolates suggested that four amino acid residues, at positions of 83, 104, and 113 in ORF 2 and position 41 in ORF 5, might be unique to some Japanese isolates.
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Demler SA, deZoeten GA (1991) The nucleotide sequence and luteovirus-like nature of RNA 1 of an aphid non-transmissible strain of pea enation mosaic virus. J Gen Virol 72:1819–1834
Fraser LR (1959) Woody gall, a suspected virus disease of rough lemon and other citrus varieties. Proc Linn Soc NSW 84:332–336
Giedroc DP, Cornish PV (2009) Frameshifting RNA pseudoknots: Structure and mechanism. Virus Res 139:193–208
Iwanami T, Kano T, Koizumi M (1992) Spherical virus-like particles associated with vein enation on Yuzu (Citrus junos Sieb. Ex Tanaka). Bull Fruit Tree Res Stn 23:137–143
Mäkinen K, Tamm T, Næss V, Truve E, Puurand Ü, Munthe T, Saarma M (1995) Characterization of cocksfoot mottle sobemovirus genomic RNA and sequence comparison with related viruses. J Gen Virol 76:2817–2825
Mayo MA, Ziegler-Graff V (1996) Molecular biology of luteovises. Adv Virus Res 56:413–460
Miller WA, Brown CM, Wang S (1997) New punctuation for the genetic code: Luteovirus gene expression. Semin Virol 8:3–13
Nakazono-Nagaoka E, Fujikawa T, Uechi N, Iwanami T (2015) Vein enation symptom in Yuzu is caused by Citrus vein enation virus [in Japanese]. Jpn J Phytopathol 81:341–345
Stevens M, Freeman B, Liu HY, Herrbach E, Lemaire O (2005) Beet poleroviruses: close friends or distant relatives? Mol Plant Pathol 6:1–9
Tanaka S, Yamada S (1961) Citrus virus diseases in Japan Proc 2nd Conf IOCV Univ Florida Press Gainesville 247–252
Vives MC, Velázquez K, Pina JA, Moreno P, Guerri J, Navarro L (2013) Identification of a new Enamovirus associated with citrus vein enation disease by deep sequencing of small RNAs. Phytopathology 103:1077–1086
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Nakazono-Nagaoka, E., Fujikawa, T. & Iwanami, T. Nucleotide sequences of Japanese isolates of citrus vein enation virus. Arch Virol 162, 879–883 (2017). https://doi.org/10.1007/s00705-016-3139-6
- Citrus Tristeza Virus
- Japanese Isolate
- Citrus Cultivar
- Serine Proteinase Domain
- Spanish Isolate