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Archives of Virology

, Volume 162, Issue 2, pp 555–559 | Cite as

Identification of a novel mycovirus isolated from Rhizoctonia solani (AG 2-2 IV) provides further information about genome plasticity within the order Tymovirales

  • Anika Bartholomäus
  • Daniel Wibberg
  • Anika Winkler
  • Alfred Pühler
  • Andreas Schlüter
  • Mark Varrelmann
Annotated Sequence Record

Abstract

The complete genome of a novel mycovirus, named Rhizoctonia solani flexivirus 1 (RsFV-1), which infects an avirulent strain of Rhizoctonia solani AG 2-2 IV, was sequenced and analyzed. Its RNA genome consists of 10,621 nucleotides, excluding the poly-A tail, and encodes a single protein of 3477 amino acids. The identification of conserved motifs of methyltransferase, helicase and RNA-dependent RNA polymerase revealed its relatedness to members of the alphavirus-like superfamily of positive-strand RNA viruses. Phylogenetic analysis of these fused domains suggested that this virus should be assigned to the order Tymovirales. The recently described Fusarium graminearum deltaflexivirus 1 was found to be its closest relative. However, the whole genome, as well as the encoded protein of RsFV-1, is larger than that of other known members of the order Tymovirales, and unlike all other viruses belonging to this order, its methyltransferase domain is not located at the N-terminus of the replicase. Although genome diversity, as a result of recombination and gene loss, is a well-documented trait in members of the order Tymovirales, no related virus with a comparable genome alteration has been reported before. For these reasons, RsFV-1 broadens our perception about genome plasticity and diversity within the order Tymovirales.

Keywords

Sugar Beet Herpes Simplex Virus Type Graminearum Helicase Domain Genome Plasticity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

This study did not include experiments with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 1 (PDF 177 kb)
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Supplementary material 2 (PDF 183 kb)
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Supplementary material 3 (PDF 103 kb)
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Supplementary material 4 (PDF 16 kb)
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Supplementary material 5 (PDF 24 kb)

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Institute of Sugar Beet ResearchGöttingenGermany
  2. 2.Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld UniversityBielefeldGermany

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