Characteristics and complete genome analysis of a novel jumbo phage infecting pathogenic Bacillus pumilus causing ginger rhizome rot disease
- 332 Downloads
Tailed phages with genomes larger than 200 kbp are classified as jumbo phage and exhibit extremely high diversity. In this study, a novel jumbo phage, vB_BpuM_BpSp, infecting pathogenic Bacillus pumilus, the cause of ginger rhizome rot disease, was isolated. Notable features of phage vB_BpuM_BpSp are the large phage capsid of 137 nm and baseplate-attached curly tail fibers. The genome of the phage is 255,569 bp in size with G+C content of 25.9 %, and it shows low similarity to known biological entities. The phage genome contains 318 predicted coding sequences. Among these predicted coding sequences, 26 genes responsible for nucleotide metabolism were found, and seven structural genes could be identified. The findings of this study provide new understanding of the genetic diversity of phages.
KeywordsPhage Genome Endolysin Tail Fiber LysM Domain Predict Code Sequence
This study was supported by the National Natural Science Foundation of China (No. 31500155, 31170123).
- 9.Hendrix RW (2009) Jumbo bacteriophages. Curr Top Microbiol 328:229–240Google Scholar
- 14.Lecoutere E, Ceyssens PJ, Miroshnikov KA, Mesyanzhinov VV, Krylov VN, Noben JP, Robben J, Hertveldt K, Volckaert G, Lavigne R (2009) Identification and comparative analysis of the structural proteomes of phi KZ and EL, two giant Pseudomonas aeruginosa bacteriophages. Proteomics 9:3215–3219CrossRefPubMedGoogle Scholar
- 22.Skurnik M, Hyytiainen HJ, Happonen LJ, Kiljunen S, Datta N, Mattinen L, Williamson K, Kristo P, Szeliga M, Kalin-Manttari L, Ahola-Iivarinen E, Kalkkinen N, Butcher SJ (2012) Characterization of the genome, proteome, and structure of yersiniophage phi R1-37. J Virol 86:12625–12642CrossRefPubMedPubMedCentralGoogle Scholar