Archives of Virology

, Volume 161, Issue 12, pp 3527–3533 | Cite as

Simultaneous mutation of G275A and P276A in the matrix protein of Newcastle disease virus decreases virus replication and budding

  • Haixu Xu
  • Zhiqiang Duan
  • Yu Chen
  • Jiajia Liu
  • Xin Cheng
  • Jingjing Liu
  • Jie Zhu
  • Xiaoquan Wang
  • Xiaowen Liu
  • Shunlin Hu
  • Xiufan Liu
Brief Report

Abstract

The matrix (M) protein of Newcastle disease virus (NDV) is a highly conserved hydrophobic viral protein. In some paramyxoviruses (measles virus and Sendai virus), the paired glycine (G) near the C terminus of the M protein may form a turn that mediates the specific interaction with the cell membrane. Similar amino acids (glycine-proline [GP], at position 275–276) exist in the M protein of NDV. However, the role of these residues in the replication and pathogenicity of NDV is unknown. In this study, recombinant NDV with the sequence GP/AA or LGP/GGL in the M protein was generated to investigate the role of this conserved sequence. Budding experiments on the mutant viruses revealed that the GP/AA mutation reduced virus budding and virus replication in DF-1 cells; biological characterization revealed attenuated virulence and pathogenicity in chickens, indicating that the GP sequence plays a critical role in the life cycle of the virus.

Keywords

Newcastle Disease Virus Mutant Virus Parental Virus Host Cell Membrane Newcastle Disease Virus Virion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank Dr. Chan Ding (Shanghai Veterinary Research Institute, China) for providing mouse anti-NDV NP monoclonal antibody (3F5).

Compliance with ethical standards

Funding

This work was supported by the Chinese Special Fund for Agro-Scientific Research in the Public Interest (201303033), the Earmarked Fund for Modern Agro-Industry Technology Research System (nycytx-41-G07), National Natural Science Foundation of China (31172338), National S&T Support Program of China (2015BAD12B03), Jiangsu Provincial Natural Science Foundation of China (BK20141273), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Conflict of interest

Both authors have declared that no conflict of interest exists.

Ethical approval

The animal research was approved by the Jiangsu Administrative Committee for Laboratory Animals (permit number: SYXKSU-2007-0005) and complied with the guidelines of Jiangsu Laboratory Animal Welfare and Ethical of Jiangsu Administrative Committee of Laboratory Animals.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Haixu Xu
    • 1
  • Zhiqiang Duan
    • 4
  • Yu Chen
    • 1
  • Jiajia Liu
    • 1
  • Xin Cheng
    • 1
  • Jingjing Liu
    • 1
  • Jie Zhu
    • 1
  • Xiaoquan Wang
    • 1
    • 2
    • 3
  • Xiaowen Liu
    • 1
    • 2
    • 3
  • Shunlin Hu
    • 1
    • 2
    • 3
  • Xiufan Liu
    • 1
    • 2
    • 3
  1. 1.College of Veterinary MedicineYangzhou UniversityYangzhouChina
  2. 2.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou UniversityYangzhouChina
  3. 3.Ministry of Educational Key Lab for Avian Preventive MedicineYangzhou UniversityYangzhouChina
  4. 4.College of Animal ScienceGuizhou UniversityGuiyangChina

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