Simultaneous mutation of G275A and P276A in the matrix protein of Newcastle disease virus decreases virus replication and budding
- 294 Downloads
The matrix (M) protein of Newcastle disease virus (NDV) is a highly conserved hydrophobic viral protein. In some paramyxoviruses (measles virus and Sendai virus), the paired glycine (G) near the C terminus of the M protein may form a turn that mediates the specific interaction with the cell membrane. Similar amino acids (glycine-proline [GP], at position 275–276) exist in the M protein of NDV. However, the role of these residues in the replication and pathogenicity of NDV is unknown. In this study, recombinant NDV with the sequence GP/AA or LGP/GGL in the M protein was generated to investigate the role of this conserved sequence. Budding experiments on the mutant viruses revealed that the GP/AA mutation reduced virus budding and virus replication in DF-1 cells; biological characterization revealed attenuated virulence and pathogenicity in chickens, indicating that the GP sequence plays a critical role in the life cycle of the virus.
KeywordsNewcastle Disease Virus Mutant Virus Parental Virus Host Cell Membrane Newcastle Disease Virus Virion
We would like to thank Dr. Chan Ding (Shanghai Veterinary Research Institute, China) for providing mouse anti-NDV NP monoclonal antibody (3F5).
Compliance with ethical standards
This work was supported by the Chinese Special Fund for Agro-Scientific Research in the Public Interest (201303033), the Earmarked Fund for Modern Agro-Industry Technology Research System (nycytx-41-G07), National Natural Science Foundation of China (31172338), National S&T Support Program of China (2015BAD12B03), Jiangsu Provincial Natural Science Foundation of China (BK20141273), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Conflict of interest
Both authors have declared that no conflict of interest exists.
The animal research was approved by the Jiangsu Administrative Committee for Laboratory Animals (permit number: SYXKSU-2007-0005) and complied with the guidelines of Jiangsu Laboratory Animal Welfare and Ethical of Jiangsu Administrative Committee of Laboratory Animals.