Archives of Virology

, Volume 161, Issue 12, pp 3509–3514 | Cite as

Ficus religiosa L. bark extracts inhibit infection by herpes simplex virus type 2 in vitro

  • Manik Ghosh
  • Andrea Civra
  • Massimo Rittà
  • Valeria Cagno
  • Siva Ganesh Mavuduru
  • Preeti Awasthi
  • David Lembo
  • Manuela DonalisioEmail author
Brief Report


Ficus religiosa extracts have been used in traditional Indian medicine to treat sexually transmitted infections such as gonorrhea and genital ulcers. The aim of this study was to investigate the antiviral activity of F. religiosa extracts against herpes simplex virus type 2 (HSV-2), the main causative agent of genital ulcers and sores. Water and chloroform bark extracts were the most active against HSV-2, and also against an acyclovir-resistant strain. We demonstrate that the water extract has a direct virus-inactivating activity. By contrast, the chloroform extract inhibits viral attachment and entry and limits the production of viral progeny.


HSV-2 Antiviral Plant extract Ficus religiosa 



This study was supported by a donation from Silvana Legnani.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

705_2016_3032_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)


  1. 1.
    Galil J (1984) Ficus religiosa L.—the tree-splitter. Bot J Linn Soc 88:185–203CrossRefGoogle Scholar
  2. 2.
    Aiyegoro OA, Okoh AI (2009) Use of bioactive plant products in combination with standard antibiotics, implications in antimicrobial chemotherapy. J Med Plants Res 3:1147–1152Google Scholar
  3. 3.
    Singh D, Singh B, Goel RK (2011) Traditional uses, phytochemistry and pharmacology of Ficus religiosa: A review. J Ethnopharm 134:565–583. doi: 10.1016/j.jep.2011.01.046 CrossRefGoogle Scholar
  4. 4.
    Choudhari AS, Suryavanshi SA, Kaul-Ghanekar R (2013) The aqueous extract of Ficus religiosa induces cell cycle arrest in human cervical cancer cell lines SiHa (HPV-16 positive) and apoptosis in HeLa (HPV-18 positive). PLoS One 8:e70127. doi: 10.1371/journal.pone.0070127 (1–10)
  5. 5.
    Kusumoto IT, Nakabayashi T, Kida H, Miyashiro H, Hattori M, Namba T (1995) Screening of various plant extracts used in ayurvedic medicine for inhibitory effects on human immunodeficiency virus type 1 (HIV-1) protease. Phytother Res 9:180–184CrossRefGoogle Scholar
  6. 6.
    Cagno V, Civra A, Kumar R, Pradhan S, Donalisio M, Nayan Sinha B, Ghosh M, Lembo D (2015) Ficus religiosa L. bark extracts inhibit human rhinovirus and respiratory syncytial virus infection in vitro. J Ethnopharmacol 176:252–257. doi: 10.1016/j.jep.2015.10.042 CrossRefPubMedGoogle Scholar
  7. 7.
    Sharma HK, Chhangte L, Dolui AK (2001) Traditional medicinal plants in Mizoram, India. Fitoterapia 72:146–161CrossRefPubMedGoogle Scholar
  8. 8.
    Roizman B, Knipe DM, Whitley RJ (2007) Herpes simplex viruses. In: Knipe DM et al (ed) Fields virology, 5th edn. Lippincott, Philadelphia, pp 2501–2601Google Scholar
  9. 9.
    Stanberry LR, Cunningham AL, Mindel A, Scott LL, Spruance SL, Aoki FY, Lacey CJ (2000) Prospects for control of herpes simplex virus disease through immunization. Clin Infect Dis 30:549–566CrossRefPubMedGoogle Scholar
  10. 10.
    Feng Z, Qiu Z, Sang Z, Lorenzo C, Glasser J (2013) Modeling the synergy between HSV-2 and HIV and potential impact of HSV-2 therapy. Math Biosci 245:171–187. doi: 10.1016/j.mbs.2013.07.003 CrossRefPubMedGoogle Scholar
  11. 11.
    Cavalli R, Donalisio M, Bisazza A, Civra A, Ranucci E, Ferruti P, Lembo D (2012) Enhanced antiviral activity of acyclovir loaded into nanoparticles. Methods Enzymol 509:1–19. doi: 10.1016/B978-0-12-391858-1.00001-0 CrossRefPubMedGoogle Scholar
  12. 12.
    Donalisio M, Cornaglia M, Landolfo S, Lembo D (2008) TGF-beta1 and IL-4 downregulate human papillomavirus-16 oncogene expression but have differential effects on the malignant phenotype of cervical carcinoma cells. Virus Res 132:253–266. doi: 10.1016/j.virusres.2007.12.003 CrossRefPubMedGoogle Scholar
  13. 13.
    Field HJ, Darby G, Wildy P (1980) Isolation and characterization of acyclovir resistant mutants of herpes simplex virus. J Gen Virol 49:115–124CrossRefPubMedGoogle Scholar
  14. 14.
    Donalisio M, Nana HM, Ngono Ngane RA, Gatsin D, Tiabou Tchinda A, Rovito R, Cagno V, Cagliero C, Boyom FF, Rubiolo P, Bicchi C, Lembo D (2013) In vitro anti-herpes simplex virus activity of crude extract of the roots of Nauclea latifolia Smith (Rubiaceae). BMC Complem Altern Med 13:266–274. doi: 10.1186/1472-6882-13-266 CrossRefGoogle Scholar
  15. 15.
    Donalisio M, Quaranta P, Chiuppesi F, Pistello M, Cagno V, Cavalli R, Volante M, Bugatti A, Rusnati M, Ranucci E, Ferruti P, Lembo D (2016) The AGMA1 poly(amidoamine) inhibits the infectivity of herpes simplex virus in cell lines, in human cervicovaginal histocultures, and in vaginally infected mice. Biomaterials 85:40–53. doi: 10.1016/j.biomaterials.2016.01.055 CrossRefPubMedGoogle Scholar
  16. 16.
    Cagno V, Donalisio M, Civra A, Cagliero C, Rubiolo P, Lembo D (2015) In vitro evaluation of the antiviral properties of Shilajit and investigation of its mechanisms of action. J Ethnopharmacol 166:129–134. doi: 10.1016/j.jep.2015.03.019 CrossRefPubMedGoogle Scholar
  17. 17.
    Civra A, Cagno V, Donalisio M, Biasi F, Leonarduzzi G, Poli G, Lembo D (2014) Inhibition of pathogenic non-enveloped viruses by 25-hydroxycholesterol and 27-hydroxycholesterol. Sci Rep 4:7487. doi: 10.1038/srep07487 CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Yarmolinsky L, Zaccai M, Ben-Shabat S, Mills D, Huleihel M (2009) Antiviral activity of ethanol extracts of Ficus binjamina and Lilium candidum in vitro. New Biotechnol 26:307–313. doi: 10.1016/j.nbt.2009.08.005 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Department of Pharmaceutical Sciences and TechnologyBirla Institute of TechnologyRanchiIndia
  2. 2.Department of Clinical and Biological Sciences, S. Luigi Gonzaga HospitalUniversity of TorinoTurinItaly

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