Isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in China
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Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated “short beak and dwarfism syndrome” (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells. Using monoclonal antibody diagnostic assays, the SBDSV M15 isolate was positive for the antigen of goose parvovirus but not Muscovy duck parvovirus. A 348-bp (2604-2951) VP1gene fragment was amplified, and its sequence indicated that the virus was most closely related to a Hungarian GPV strain that was also isolated from mule ducks with SBDS disease. A similar disease was reproduced by inoculating birds with SBDSV M15. Together, these data indicate that SBDSV M15 is a GPV-related parvovirus causing SBDS disease and that it is divergent from classical GPV isolates.
KeywordsLatex Agglutination Allantoic Fluid Muscovy Duck Blind Passage Duck Embryo
Compliance with ethical standards
This study was funded by grants from the Natural Science Foundation of China (U1305212, 31402236), the Youth Elite Project of Fujian Academy of Agriculture Science (YC2016-1), the Fujian Public Welfare Project (2014R1023-1), the Fujian Major Platform of Livestock and Poultry Disease Prevention and Control Technology (2014N2003) and the National Waterfowl Industry and Technology System of China (CARS-43-01A).
Conflict of interest
The authors declare that they have no conflict of interest.
The animal protocol used in this study was approved by the Research Ethics Committee of the Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences. All experimental procedures involving ducks were performed in accordance with the Regulations of the Administration of Affairs Concerning Experimental Animals, approved by the State Council of China.
- 3.Dressy D (1967) A viral disease of goslings. Acta Vet Hung 17:443–448Google Scholar
- 6.Glávits R, Zolnai A, Szabó É, Ivanics É, Zarka P, Mató T, Palya V (2005) Comparative pathological studies on domestic geese (Anser anser domestica) and Muscovy ducks (Cairina moschata) experimentally infected with parvovirus strains of goose and Muscovy duck origin. Acta Vet Hung 53:73–89CrossRefPubMedGoogle Scholar
- 7.Cheng YQ, Lin TL, Hu QL, Li YY, Zhou WM, Wu ZY (1993) Isolation and identification of a Muscovy duckling parvovirus. Chin J Virol 3:228–235Google Scholar
- 8.Woźniakowski G, Samorek-Salamonowicz E, Kozdruń W (2012) Quantitative analysis of waterfowl parvoviruses in geese and Muscovy ducks by real-time polymerase chain reaction: correlation between age, clinical symptoms and DNA copy number of waterfowl parvoviruses. BMC Vet Res 8:1–10CrossRefGoogle Scholar
- 13.Cheng YQ, Hu QL, Li YY, Chen SY, Lin TL (1997) Studies on diagnostic techniques and reagents of Muscovy Duckling parvovirusis. Chin J Vet Sci 5:434–436Google Scholar
- 14.Zhu X, Chen S, Lin F, Cheng X, Chen S, Wang S, Li Z (2012) Development of the latex agglutination diagnostic method of muscovy duck gosling plague. Chin J Prev Vet Med 34(9):715–718Google Scholar
- 15.Zhu XL, Chen SY, Cheng XX, Lin Q, Chen SL, Wang S, LI ZL, Wang YL (2012) Praparation for direct fluorescence diagnosis reagent on Muscovy duck gosling plague. J Anim Sci Vet Med 31(4):1–3 (in Chinese) Google Scholar