Analysis of the coding-complete genomic sequence of groundnut ringspot virus suggests a common ancestor with tomato chlorotic spot virus
Groundnut ringspot virus (GRSV) and tomato chlorotic spot virus (TCSV) share biological and serological properties, so their identification is carried out by molecular methods. Their genomes consist of three segmented RNAs: L, M and S. The finding of a reassortant between these two viruses may complicate correct virus identification and requires the characterization of the complete genome. Therefore, we present for the first time the complete sequences of all the genes encoded by a GRSV isolate. The high level of sequence similarity between GRSV and TCSV (over 90 % identity) observed in the genes and proteins encoded in the M RNA support previous results indicating that these viruses probably have a common ancestor.
KeywordsReverse Transcription Polymerase Chain Reaction Tomato Spotted Wilt Virus Reassortment Event Tomato Spotted Wilt Virus Isolate Tospovirus Species
Compliance with ethical standards
I have read and have abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.
This work was supported by Fundación Maní Argentino and the PNIND PE 1108072 project of Instituto Nacional de Tecnología Agropecuaria (INTA).
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Ananthakrishnan TN, Annadurai RS (2007) Thrips–tospovirus interactions: biological and molecular implications. Curr Sci 92:1083–1086Google Scholar
- 18.Plyusnin A, Beaty BJ, Elliott RM, Goldbach R, Kormelink R, Lundkvist A, Schmaljohn CS, Tesh RB (2012) Family Bunyaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier Inc, London, pp 725–741Google Scholar
- 19.Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- 22.Soellick T-R, Uhrig JF, Bucher GL, Kellmann J-W, Schreier PH (2000) The movement protein NSm of tomato spotted wilt topovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting proteins. Proc Natl Acad Sci USA 97:2373–2378CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Sundaraj S, Srinivasan R, Culbreath AK, Riley DG, Pappu HR (2014) Host plant resistance against Tomato spotted wilt virus in peanut (Arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival. Phytopathology 104:202–210CrossRefPubMedGoogle Scholar
- 29.Webster CG, Frantz G, Reitz SR, Funderburk JE, Mellinger HC, McAvoy E, Turechek WW, Marshall SH, Tantiwanich Y, McGrath MT, Daughtrey ML, Adkins S (2015) Emergence of groundnut ringspot virus and tomato chlorotic spot virus in vegetables in Florida and the Southeastern United States. Phytopatology 105:388–398CrossRefGoogle Scholar