Archives of Virology

, Volume 161, Issue 8, pp 2311–2316 | Cite as

Analysis of the coding-complete genomic sequence of groundnut ringspot virus suggests a common ancestor with tomato chlorotic spot virus

  • Soledad de BreuilEmail author
  • Joaquín Cañizares
  • José Miguel Blanca
  • Nicolás Bejerman
  • Verónica Trucco
  • Fabián Giolitti
  • Peio Ziarsolo
  • Sergio Lenardon
Brief Report


Groundnut ringspot virus (GRSV) and tomato chlorotic spot virus (TCSV) share biological and serological properties, so their identification is carried out by molecular methods. Their genomes consist of three segmented RNAs: L, M and S. The finding of a reassortant between these two viruses may complicate correct virus identification and requires the characterization of the complete genome. Therefore, we present for the first time the complete sequences of all the genes encoded by a GRSV isolate. The high level of sequence similarity between GRSV and TCSV (over 90 % identity) observed in the genes and proteins encoded in the M RNA support previous results indicating that these viruses probably have a common ancestor.


Reverse Transcription Polymerase Chain Reaction Tomato Spotted Wilt Virus Reassortment Event Tomato Spotted Wilt Virus Isolate Tospovirus Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Compliance with ethical standards

I have read and have abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.


This work was supported by Fundación Maní Argentino and the PNIND PE 1108072 project of Instituto Nacional de Tecnología Agropecuaria (INTA).

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Almeida MMS, Orílio AF, Melo FL, Rodriguez R, Feliz A, Cayetano X, Martínez RT, Resende RO (2014) The first report of tomato chlorotic spot virus (TCSV) infecting long beans and chili peppers in the dominican republic. Plant Dis 98:1285CrossRefGoogle Scholar
  2. 2.
    Ananthakrishnan TN, Annadurai RS (2007) Thrips–tospovirus interactions: biological and molecular implications. Curr Sci 92:1083–1086Google Scholar
  3. 3.
    Blanca JM, Pascual L, Ziarsolo P, Nuez F, Cañizares J (2011) ngs_backbone: a pipeline for read cleaning, mapping and snp calling using next generation sequence. BMC Genom 12:285CrossRefGoogle Scholar
  4. 4.
    Boari AJ, Maciel-Zambolim E, Lau DD, Lima GSA, Kitajima EW, Brommonschenkel SH, Zerbini FM (2002) Detection and partial characterization of an isolate of groundnut ringspot virus in Solanum sessiliflorum. Fitopatol Bras 27:249–253CrossRefGoogle Scholar
  5. 5.
    Briese T, Calisher CH, Higgs S (2013) Viruses of the family Bunyaviridae: are all available isolates reassortants? Virology 446:207–216CrossRefPubMedGoogle Scholar
  6. 6.
    Hagen C, Frizzi A, Kao J, Jia L, Huang M, Zhang Y, Huang S (2011) Using small RNA sequences to diagnose, sequence, and investigate the infectivity characteristics of vegetable-infecting viruses. Arch Virol 156:1209–1216CrossRefPubMedGoogle Scholar
  7. 7.
    Hogenhout SA, Ammar E-D, Whitfield AE, Redinbaugh MG (2008) Insect vector interactions with persistently transmitted viruses. Annu Rev Phytopathol 46:327–359CrossRefPubMedGoogle Scholar
  8. 8.
    Kreuze JF, Perez A, Untiveros M, Quispe D, Fuentes S, Barker I, Simon R (2009) Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: a generic method for diagnosis, discovery and sequencing of viruses. Virology 388:1–7CrossRefPubMedGoogle Scholar
  9. 9.
    Law MD, Moyer JW (1990) A tomato spotted wilt-like virus with a serologically distinct N protein. J Gen Virol 71:933–938CrossRefGoogle Scholar
  10. 10.
    Lewandowskia DJ, Adkins S (2005) The tubule-forming NSm protein from tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids. Virology 342:26–37CrossRefGoogle Scholar
  11. 11.
    Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth GT, Abecasis GR, Durbin R (2009) The sequence alignment/map format and SAMtools. Bioinformatics 25:2078–2079CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Lian S, Lee JS, Cho WK, Yu J, Kim MK, Choi HS, Kim KH (2013) Phylogeneticand recombination analysis of tomato spotted wilt virus. PLOS ONE 8:e63380. doi: 10.1371/journal.pone.0063380 CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Londoño A, Capobianco H, Zhang S, Polston JE (2012) First record of tomato chlorotic spot virus in the USA. Trop Plant Pathol 37:333–338CrossRefGoogle Scholar
  14. 14.
    Lovato FA, Nagata T, de Oliveira Resende R, de Avila AC, Inoue-Nagata AK (2004) Sequence analysis of the glycoproteins of tomato chlorotic spot virus and groundnut ringspot virus and comparison with other tospoviruses. Virus Genes 29:321–328CrossRefPubMedGoogle Scholar
  15. 15.
    Margaria P, Ciuffo M, Rosa C, Turina M (2015) Evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates. Virus Res 196:157–161CrossRefPubMedGoogle Scholar
  16. 16.
    Milne I, Stephen G, Bayer M, Cock PJA, Pritchard L, Cardle L, Shaw PD, Marshall D (2013) Using tablet for visual exploration of second-generation sequencing data. Brief Bioinform 14:193–202CrossRefPubMedGoogle Scholar
  17. 17.
    Pappu HR, Jones RAC, Jain RK (2009) Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Res 141:219–236CrossRefPubMedGoogle Scholar
  18. 18.
    Plyusnin A, Beaty BJ, Elliott RM, Goldbach R, Kormelink R, Lundkvist A, Schmaljohn CS, Tesh RB (2012) Family Bunyaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier Inc, London, pp 725–741Google Scholar
  19. 19.
    Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  20. 20.
    Silva MS, Martins CRF, Bezerra IC, Nagata T, de Ávila AC, Resende RO (2001) Sequence diversity of NSm movement proteins of tospoviruses. Arch Virol 146:1267–1281CrossRefPubMedGoogle Scholar
  21. 21.
    Sin SH, McNulty BC, Kennedy GG, Moyer JW (2005) Viral genetic determinants for thrips transmission of Tomato spotted wilt virus. Proc Natl Acad Sci USA 102:5168–5173CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Soellick T-R, Uhrig JF, Bucher GL, Kellmann J-W, Schreier PH (2000) The movement protein NSm of tomato spotted wilt topovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting proteins. Proc Natl Acad Sci USA 97:2373–2378CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Sundaraj S, Srinivasan R, Culbreath AK, Riley DG, Pappu HR (2014) Host plant resistance against Tomato spotted wilt virus in peanut (Arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival. Phytopathology 104:202–210CrossRefPubMedGoogle Scholar
  24. 24.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Tentchev D, Verdin E, Marchal C, Jacquet M, Aguilar JM, Moury B (2011) Evolution and structure of tomato spotted wilt virus populations: evidence of extensive reassortment and insights into emergence processes. J Gen Virol 92:961–973CrossRefPubMedGoogle Scholar
  26. 26.
    Timmerman-Vaughan GM, Lister R, Cooper R, Tang J (2014) Phylogenetic analysis of New Zealand tomato spotted wilt virus isolates suggests likely incursion history scenarios and mechanisms for population evolution. Arch Virol 159:993–1003CrossRefPubMedGoogle Scholar
  27. 27.
    Tsompana M, Moyer JW (2008) Tospoviruses. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of virology, vol 5, 3rd edn. Elsevier Ltd, Oxford, pp 157–162CrossRefGoogle Scholar
  28. 28.
    Webster CG, Reitz SR, Perry KL, Adkins S (2011) A natural M RNA reassortant arising from two species of plant- and insect-infecting bunyaviruses and comparison of its sequence and biological properties to parental species. Virology 413:216–225CrossRefPubMedGoogle Scholar
  29. 29.
    Webster CG, Frantz G, Reitz SR, Funderburk JE, Mellinger HC, McAvoy E, Turechek WW, Marshall SH, Tantiwanich Y, McGrath MT, Daughtrey ML, Adkins S (2015) Emergence of groundnut ringspot virus and tomato chlorotic spot virus in vegetables in Florida and the Southeastern United States. Phytopatology 105:388–398CrossRefGoogle Scholar
  30. 30.
    Wu Q, Luo Y, Lu R, Lau N, Lai EC, Li W-X, Ding S-W (2010) Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs. Proc Natl Acad Sci USA 107:1606–1611CrossRefPubMedPubMedCentralGoogle Scholar
  31. 31.
    Zerbino DR, Birney E (2008) Velvet: algorithms for de novo read assembly using de Bruijn graphs. Genome Res 18:821–829CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Soledad de Breuil
    • 1
    • 2
    Email author
  • Joaquín Cañizares
    • 3
  • José Miguel Blanca
    • 3
  • Nicolás Bejerman
    • 1
    • 2
  • Verónica Trucco
    • 1
  • Fabián Giolitti
    • 1
  • Peio Ziarsolo
    • 3
  • Sergio Lenardon
    • 1
  1. 1.Instituto de Patología Vegetal, Centro de Investigaciones AgropecuariasInstituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA)CórdobaArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)CABAArgentina
  3. 3.Instituto de Conservación y Mejora de la Agrodiversidad ValencianaUniversitat Politècnica de Valencia (COMAV-UPV)ValenciaSpain

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