Molecular characteristics of a novel strain of canine minute virus associated with hepatitis in a dog
- 274 Downloads
- 2 Citations
Abstract
A 5-year-old female Yorkshire terrier dog died a few days following hernia and ovariohysterectomy surgeries. Necropsy performed on the dog revealed that the surgeries were not the cause of death; however, degenerative viral hepatitis, showing intranuclear inclusion bodies in hepatic cells, was observed in histopathologic examination. Several diagnostic methods were used to screen for the cause of disease, and minute virus of canines (MVC) was detected in all parenchymal organs, including the liver. Other pathogens that may cause degenerative viral hepatitis were not found. Infection with MVC was confirmed by in situ hybridization, which revealed the presence of MVC nucleic acid in the liver tissue of the dog. Through sequencing and phylogenetic analysis of the nearly complete genome sequence, the strain was found to be distinct from other previously reported MVC strains. These results indicate that this novel MVC strain might be related to degenerative viral hepatitis in dogs.
Keywords
Minute virus of canines Canine Hepatitis Phylogenetic analysisNotes
Acknowledgments
This research was supported by a grant from the Animal and Plant Quarantine Agency, Anyang, Gyeonggi, Republic of Korea.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any animal studies that were performed by any of the authors.
References
- 1.Binn LN, Lazar EC, Eddy GA, Kajima M (1970) Recovery and characterization of a minute virus of canines. Infect Immun 1:503–508PubMedPubMedCentralGoogle Scholar
- 2.Bodewes R, Lapp S, Hahn K, Habierski A, Forster C, Konig M, Wohlsein P, Osterhaus ADME, Baumgartner W (2014) Novel canine bocavirus strain associated with severe enteritis in a dog litter. Vet Microbiol 174:1–8CrossRefPubMedGoogle Scholar
- 3.Brown KE (2010) The expanding range of parvoviruses which infect humans. Rev Med Virol 20:231–244CrossRefPubMedGoogle Scholar
- 4.Carmichael LE, Schlafer DH, Hashimoto A (1991) Pathogenicity of minute virus of canines (MVC) for the canine fetus. Cornell Vet 81:151–171PubMedGoogle Scholar
- 5.Carmichael LE, Schlafer DH, Hashimoto A (1994) Minute virus of canines (MVC, canine parvovirus type-1): pathogenicity for pups and seroprevalence estimate. J Vet Diagn Invest 6:165–174CrossRefPubMedGoogle Scholar
- 6.Decaro N, Amorisco F, Lenoci D, Lovero A, Colaianni ML, Losurdo M, Desario C, Martella V, Buonavoglia C (2012) Molecular characterization of Canine minute virus associated with neonatal mortality in a litter of Jack Russell terrier dogs. J Vet Diagn Invest 24:755–758CrossRefPubMedGoogle Scholar
- 7.Eminaga S, Palus V, Cherubini GB (2011) Minute virus as a possible cause of neurological problems in dogs. Vet Rec 168:111–112CrossRefPubMedGoogle Scholar
- 8.Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, CaliforniaGoogle Scholar
- 9.Jang HK, Tohya Y, Han KY, Kim TJ, Song CS, Mochizuki M (2003) Seroprevalence of canine calicivirus and canine minute virus in the Republic of Korea. Vet Rec 153:150–152CrossRefPubMedGoogle Scholar
- 10.Kainulainen L, Waris M, Soderlund-Venermo M, Allander T, Hedman K, Ruuskanen O (2008) Hepatitis and human bocavirus primary infection in a child with T-cell deficiency. J Clin Microbiol 46:4104–4105CrossRefPubMedPubMedCentralGoogle Scholar
- 11.Li L, Pesavento PA, Leutenegger CM, Estrada M, Coffey LL, Naccache SN, Samayoa E, Chiu C, Qiu J, Wang C, Deng X, Delwart E (2013) A novel bocavirus in canine liver. Virol J 10:54CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Manteufel J, Truyen U (2008) Animal bocaviruses: a brief review. Intervirology 51:328–334CrossRefPubMedGoogle Scholar
- 13.Mochizuki M, Hashimoto M, Hajima T, Takiguchi M, Hashimoto A, Une Y, Roerink F, Ohshima T, Parrish CR, Carmichael LE (2002) Virologic and serologic identification of minute virus of canines (canine parvovirus type 1) from dogs in Japan. J Clin Microbiol 40:3993–3998CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Ohshima T, Kishi M, Mochizuki M (2004) Sequence analysis of an Asian isolate of minute virus of canines (canine parvovirus type 1). Virus Genes 29:291–296CrossRefPubMedGoogle Scholar
- 15.Ohshima T, Kawakami K, Abe T, Mochizuki M (2010) A minute virus of canines (MVC: canine bocavirus) isolated from an elderly dog with severe gastroenteritis, and phylogenetic analysis of MVC strains. Vet Microbiol 145:334–338CrossRefPubMedGoogle Scholar
- 16.Schwartz D, Green B, Carmichael LE, Parrish CR (2002) The canine minute virus (minute virus of canines) is a distinct parvovirus that is most similar to bovine parvovirus. Virology 302:219–223CrossRefPubMedGoogle Scholar
- 17.Shan TL, Cui L, Dai XQ, Guo W, Shang XG, Yu Y, Zhang W, Kang YJ, Shen Q, Yang ZB, Zhu JG, Hua XG (2010) Sequence analysis of an isolate of minute virus of canines in China reveals the closed association with bocavirus. Mol Biol Rep 37:2817–2820CrossRefPubMedGoogle Scholar
- 18.Spahn GJ, Mohanty SB, Hetrick FM (1966) Experimental infection of calves with hemadsorbing enteric (HADEN) virus. Cornell Vet 56:377–386PubMedGoogle Scholar
- 19.Storz J, Leary JJ, Carlson JH, Bates RC (1978) Parvoviruses associated with diarrhea in calves. J Am Vet Med Assoc 173:624–627PubMedGoogle Scholar