Archives of Virology

, Volume 161, Issue 6, pp 1715–1717 | Cite as

Complete genome sequence of yam chlorotic necrotic mosaic virus from Dioscorea parviflora

  • Pengyuan Zhang
  • Jiejun Peng
  • Huachun Guo
  • Jianping Chen
  • Suiyun Chen
  • Jianguang Wang
Annotated Sequence Record


The complete genome sequence of yam chlorotic necrotic mosaic virus (YCNMV) was determined. It is a monopartite ssRNA 8208 nucleotides in length (excluding the poly(A) tail) and encoding a polyprotein of 2622 amino acids. Sequence analysis showed that the P1 region and some conserved motifs, such as the typical potyvirus aphid-transmission motifs DAG, PTK and KITC, are absent. Phylogenetic analysis based on the complete polyprotein sequences of YCNMV and selected members of the family Potyviridae clearly showed that this virus should be assigned to the genus Macluravirus and suggest that YCNMV is a new member of the genus Macluravirus.


Yunnan Province Complete Genome Sequence Complete Nucleotide Sequence Accession Number KT724961 Chlorosis Symptom 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by a research grant from the National Natural Science Foundation of China (Grant 31101416) and Foundation of the Life Science College of Yunnan University (Grant 2013S205). We thank Professor Michael J. Adams (Rothamsted Research, Harpenden, UK) for his valuable advice and critical editing of the manuscript.

Compliance with ethical standards


This study was funded by a research grant from the National Natural Science Foundation of China (Grant 31101416) and Foundation of the Life Science College of Yunnan University (Grant 2013S205).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2016_2818_MOESM1_ESM.doc (256 kb)
Supplementary material 1 (DOC 256 kb)
705_2016_2818_MOESM2_ESM.doc (30 kb)
Supplementary material 2 (DOC 30 kb)


  1. 1.
    Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479CrossRefPubMedGoogle Scholar
  2. 2.
    Adams MJ, Zerbini FM, French R, Rabenstein F, Stenger DC, Valkonen JPT (2012) Family Potyviridae. In: King MQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, pp 1069–1089Google Scholar
  3. 3.
    Chen J, Chen JP (2002) Determination of genome sequence of potyviruses by degenerated PCR and RACE methods. Chin J Virol 18:371–374Google Scholar
  4. 4.
    Chen J, Chen J, Adams MJ (2001) A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Arch Virol 146:757–766CrossRefPubMedGoogle Scholar
  5. 5.
    Kondo T (2001) The 3′-terminal sequence of Chinese yam necrotic mosaic virus genomic RNA: a close relationship with macluravirus. Arch Virol 146:1527–1535CrossRefPubMedGoogle Scholar
  6. 6.
    Kondo T, Fujita T (2012) Complete nucleotide sequence and construction of an infectious clone of Chinese yam necrotic mosaic virus suggest that macluraviruses have the smallest genome among members of the family Potyviridae. Arch Virol 157:2299–2307CrossRefPubMedGoogle Scholar
  7. 7.
    Minutillo SA, Marais A, Mascia T, Faure C, Svanella-Dumas L, Theil S, Payet A, Perennec S, Schoen L, Gallitelli D, Candresse T (2015) Complete nucleotide sequence of Artichoke latent virus shows it to be a member of the genus Macluravirus in the family Potyviridae. Phytopathology 105:1155–1160CrossRefPubMedGoogle Scholar
  8. 8.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Wang JG, Zou XJ, Zheng HY, Adams MJ, Chen HR, Chen JP (2009) Molecular characterization of a new macluravirus from yam in Yunnan, China. Arch Virol 154:1379–1380CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Pengyuan Zhang
    • 1
  • Jiejun Peng
    • 2
  • Huachun Guo
    • 3
  • Jianping Chen
    • 2
  • Suiyun Chen
    • 1
  • Jianguang Wang
    • 1
  1. 1.The Life Science College of Yunnan UniversityKunmingChina
  2. 2.Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhouChina
  3. 3.College of Agronomy and Biological TechnologyYunnan Agricultural UniversityKunmingChina

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