Complete genome sequence of tobacco virus 1, a closterovirus from Nicotiana tabacum
- 217 Downloads
The complete genome sequence of a novel virus, provisionally named tobacco virus 1 (TV1), was determined, and this virus was identified in leaves of tobacco (Nicotiana tabacum) exhibiting leaf mosaic and yellowing symptoms in Anhui Province, China. The genome sequence of TV1 consists of 15,395 nucleotides with 61.6 % nucleotide sequence identity to mint virus 1 (MV1). Its genome organization is similar to that of MV1, containing nine open reading frames (ORFs) that potentially encode proteins with putative functions in virion assembly, cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis of the heat shock protein 70 homolog (HSP70h) placed TV1 alongside members of the genus Closterovirus in the family Closteroviridae. To our knowledge, this study is the first report of the complete genome sequence of a closterovirus identified in tobacco.
This study was supported by Key Program of Anhui Province Tobacco Monopoly Administration (no. 20150551007) and the Chinese National Natural Science Foundation (no. 31272011).
- 7.Ding M, Yang C, Zhang L, Jiang ZL, Fang Q, Qin XY, Zhang ZK (2011) Occurrence of Chilli veinal mottle virus in Nicotiana tabacum in Yunnan, China. Plant Dis 95:357Google Scholar
- 10.Dong JH, Yin YY, Xu XY, Duan YM, Zhang ZK (2010) First report of tomato spotted wilt virus in tomato and tobacco in China. J Plant Pathol 92:S121–S121Google Scholar
- 15.Martelli GP, Agranovsky AA, Bar-Joseph M, Boscia D, Candresse T, Coutts RH, Dolja VV, Hu JS, Jelkmann W, Karasev AV, Martin RR, Minafra A, Namba S, Vetten HJ (2012) Family Closteroviridae. In: King A, Adams MJ, Carstens EB, Lefkowitz E (eds) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses. Elsevier-Academic Press, San Diego, pp 987–1001Google Scholar