Archives of Virology

, Volume 161, Issue 4, pp 1087–1090 | Cite as

Complete genome sequence of tobacco virus 1, a closterovirus from Nicotiana tabacum

  • Fang Wang
  • Shuishui Qi
  • Zhengliang Gao
  • Ibukun A. Akinyemi
  • Dafeng Xu
  • Benguo Zhou
Annotated Sequence Record

Abstract

The complete genome sequence of a novel virus, provisionally named tobacco virus 1 (TV1), was determined, and this virus was identified in leaves of tobacco (Nicotiana tabacum) exhibiting leaf mosaic and yellowing symptoms in Anhui Province, China. The genome sequence of TV1 consists of 15,395 nucleotides with 61.6 % nucleotide sequence identity to mint virus 1 (MV1). Its genome organization is similar to that of MV1, containing nine open reading frames (ORFs) that potentially encode proteins with putative functions in virion assembly, cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis of the heat shock protein 70 homolog (HSP70h) placed TV1 alongside members of the genus Closterovirus in the family Closteroviridae. To our knowledge, this study is the first report of the complete genome sequence of a closterovirus identified in tobacco.

References

  1. 1.
    Agranovsky AA, Koonin EV, Boyko VP, Maiss E, Frotschl R, Lunina NA, Atabekov JG (1994) Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease. Virology 198:311–324CrossRefPubMedGoogle Scholar
  2. 2.
    Agranovsky AA, Lesemann DE, Maiss E, Hull R, Atabekov JG (1995) “Rattlesnake” structure of a filamentous plant RNA virus built of two capsid proteins. Proc Natl Acad Sci USA 92:2470–2473CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Alzhanova DV, Hagiwara Y, Peremyslov VV, Dolja VV (2000) Genetic analysis of the cell-to-cell movement of beet yellows closterovirus. Virology 268:192–200CrossRefPubMedGoogle Scholar
  4. 4.
    Alzhanova DV, Napuli AJ, Creamer R, Dolja VV (2001) Cell-to-cell movement and assembly of a plant closterovirus: roles for the capsid proteins and Hsp70 homolog. EMBO J 20:6997–7007CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Alzhanova DV, Prokhnevsky AI, Peremyslov VV, Dolja VV (2007) Virion tails of beet yellows virus: coordinated assembly by three structural proteins. Virology 359:220–226CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Chatzivassiliou EK, Katis NI, Tchomguia M, Peters D (1999) A closterovirus (family: Closteroviridae) isolated from tobacco crops in Northern Greece (Macedonia). Plant Dis 83:198CrossRefGoogle Scholar
  7. 7.
    Ding M, Yang C, Zhang L, Jiang ZL, Fang Q, Qin XY, Zhang ZK (2011) Occurrence of Chilli veinal mottle virus in Nicotiana tabacum in Yunnan, China. Plant Dis 95:357Google Scholar
  8. 8.
    Dolja VV (2003) Beet yellows virus: the importance of being different. Mol Plant Pathol 4:91–98CrossRefPubMedGoogle Scholar
  9. 9.
    Dolja VV, Kreuze JF, Valkonen JPT (2006) Comparative and functional genomics of closteroviruses. Virus Res 117:38–51CrossRefPubMedGoogle Scholar
  10. 10.
    Dong JH, Yin YY, Xu XY, Duan YM, Zhang ZK (2010) First report of tomato spotted wilt virus in tomato and tobacco in China. J Plant Pathol 92:S121–S121Google Scholar
  11. 11.
    Karasev AV (2000) Genetic diversity and evolution of closteroviruses. Annu Rev Phytopathol 38:293–324CrossRefPubMedGoogle Scholar
  12. 12.
    Kreuze JF, Perez A, Untiveros M, Quispe D, Fuentes S, Barker I, Simon R (2009) Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: a generic method for diagnosis, discovery and sequencing of viruses. Virology 388:1–7CrossRefPubMedGoogle Scholar
  13. 13.
    Letunic I, Doerks T, Bork P (2015) SMART: recent updates, new developments and status in 2015. Nucleic Acids Res 43:D257–D260CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Lu XP, Gui YJ, Xiao BG, Li YP, Tong ZJ, Liu Y, Bai XF, Wu WR, Xia L, Huttner E, Kilian A, Fan LJ (2013) Development of DArT markers for a linkage map of flue-cured tobacco. Chin Sci Bull 58:641–648CrossRefGoogle Scholar
  15. 15.
    Martelli GP, Agranovsky AA, Bar-Joseph M, Boscia D, Candresse T, Coutts RH, Dolja VV, Hu JS, Jelkmann W, Karasev AV, Martin RR, Minafra A, Namba S, Vetten HJ (2012) Family Closteroviridae. In: King A, Adams MJ, Carstens EB, Lefkowitz E (eds) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses. Elsevier-Academic Press, San Diego, pp 987–1001Google Scholar
  16. 16.
    Menzel W, Goetz R, Lesemann DE, Vetten HJ (2009) Molecular characterization of a closterovirus from carrot and its identification as a German isolate of carrot yellow leaf virus. Arch Virol 154:1343–1347CrossRefPubMedGoogle Scholar
  17. 17.
    Napuli AJ, Alzhanova DV, Doneanu CE, Barofsky DF, Koonin EV, Dolja VV (2003) The 64-kilodalton capsid protein homolog of beet yellows virus is required for assembly of virion tails. J Virol 77:2377–2384CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Peremyslov VV, Pan YW, Dolja VV (2004) Movement protein of a closterovirus is a type III integral transmembrane protein localized to the endoplasmic reticulum. J Virol 78:3704–3709CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Satyanarayana T, Gowda S, Ayllon MA, Dawson WO (2004) Closterovirus bipolar virion: evidence for initiation of assembly by minor coat protein and its restriction to the genomic RNA 5’ region. Proc Natl Acad Sci USA 101:799–804CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Tian TY, Rubio L, Yeh HH, Crawford B, Falk BW (1999) Lettuce infectious yellows virus: in vitro acquisition analysis using partially purified virions and the whitefly Bemisia tabaci. J Gen Virol 80:1111–1117CrossRefPubMedGoogle Scholar
  22. 22.
    Tian YP, Liu JL, Zhang CL, Liu YY, Wang B, Li XD, Guo ZK, Valkonen JPT (2011) Genetic diversity of potato virus Y infecting tobacco crops in China. Phytopathology 101:377–387CrossRefPubMedGoogle Scholar
  23. 23.
    Tzanetakis IE, Postman JD, Martin RR (2005) Characterization of a novel member of the family Closteroviridae from Mentha spp. Phytopathology 95:1043–1048CrossRefPubMedGoogle Scholar
  24. 24.
    Wu Q, Luo Y, Lu R, Lau N, Lai EC, Li WX, Ding SW (2010) Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs. Proc Natl Acad Sci USA 107:1606–1611CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Fang Wang
    • 1
  • Shuishui Qi
    • 2
  • Zhengliang Gao
    • 1
  • Ibukun A. Akinyemi
    • 2
  • Dafeng Xu
    • 1
  • Benguo Zhou
    • 1
  1. 1.Tobacco Research InstituteAnhui Academy of Agricultural SciencesHefeiChina
  2. 2.School of Life SciencesUniversity of Science and Technology of ChinaHefeiChina

Personalised recommendations