Archives of Virology

, Volume 161, Issue 4, pp 977–980 | Cite as

Multiple amino acid substitutions involved in the adaptation of avian-origin influenza A (H10N7) virus in mice

  • Haibo Wu
  • Xiuming Peng
  • Xiaorong Peng
  • Linfang Cheng
  • Changzhong Jin
  • Xiangyun Lu
  • Tiansheng Xie
  • Hangping Yao
  • Nanping WuEmail author
Brief Report


To identify substitutions that are possibly associated with the adaptation of avian-origin H10N7 virus to mammals, adaptation of the H10N7 virus in mouse lung was carried out by serial lung-to-lung passage. Genomic analysis of the mouse-adapted virus revealed amino acid changes in the PB2 (E627K), PA (T97I), and HA (G409E) proteins, and this virus was more virulent in mice than the wild-type virus. Our results suggest that these substitutions are involved in the enhancement of the replication efficiency of avian-origin H10N7 virus, resulting in severe disease in mice. Continued poultry surveillance of these substitutions in H10N7 viruses is required.


Avian influenza viruses Subtype H10N7 Mice Adaptation Virulence Replication 



This study was supported by Grants from the National Science Foundation of the People’s Republic of China (81502852), Zhejiang Provincial Natural Science Foundation of China (Y15H190006), and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2014ZZ12 and 2015ZZ05).

Supplementary material

705_2015_2722_MOESM1_ESM.doc (78 kb)
Supplementary material 1 (DOC 77 kb)


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Haibo Wu
    • 1
  • Xiuming Peng
    • 1
  • Xiaorong Peng
    • 1
  • Linfang Cheng
    • 1
  • Changzhong Jin
    • 1
  • Xiangyun Lu
    • 1
  • Tiansheng Xie
    • 1
  • Hangping Yao
    • 1
  • Nanping Wu
    • 1
    Email author
  1. 1.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of MedicineZhejiang UniversityHangzhouChina

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