Occurrence and characterization of plum pox virus strain D isolates from European Russia and Crimea
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Numerous plum pox virus (PPV) strain D isolates have been found in geographically distant regions of European Russia and the Crimean peninsula on different stone fruit hosts. Phylogenetic analysis of their partial and complete genomes suggests multiple introductions of PPV-D into Russia. Distinct natural isolates from Prunus tomentosa were found to bear unique amino acid substitutions in the N-terminus of the coat protein (CP) that may contribute to the adaptation of PPV-D to this host. Serological analysis using the PPV-D-specific monoclonal antibody 4DG5 provided further evidence that mutations at positions 58 and 59 of the CP are crucial for antibody binding.
KeywordsPlum pox virus Strain D 454 Pyrosequencing Phylogenetic analysis Serological analysis
We gratefully acknowledge Drs. Anatoly Yushev and Olga Radchenko (Vavilov Research Institute of Plant Industry) for their cooperation in the Pavlovsk Research Station stone fruit collection survey and for helpful discussions. We thank Dr. Delano James for the serological analysis of the isolate Vulcan. This work was supported by the Russian Scientific Foundation (research project no. 14-24-00007).
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