Archives of Virology

, Volume 160, Issue 11, pp 2741–2748 | Cite as

A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions of immunoprotective epitopes in the capsid protein

  • Rafael Locatelli Salgado
  • Pedro Marcus Pereira Vidigal
  • Natalia F. Gonzaga
  • Luiz F. L. de Souza
  • Marcelo D. Polêto
  • Thiago Souza Onofre
  • Monique R. Eller
  • Carlos Eduardo Real Pereira
  • Juliana L. R. Fietto
  • Gustavo C. Bressan
  • Roberto M. C. Guedes
  • Márcia R. Almeida
  • Abelardo Silva Júnior
Original Article


Porcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea. Here, we show that these isolates share a combination of low-frequency substitutions (single amino acid polymorphisms with a frequency of ≤25 %) in the viral capsid protein, mainly in regions of immunoprotective epitopes, and an additional lysine residue at position 234. These isolates were phylogenetically grouped in the PCV2b clade, reinforcing the idea of the emergence of a new group of mutants PCV2b associated with outbreaks worldwide. The identification of these polymorphisms in the viral capsid highlights the importance of considering these isolates for the development of more-effective vaccines.


Capsid Protein Viral Capsid Conformational Epitope Viral Capsid Protein Classical Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by grants from the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The funders had no role in the study design, data collection, analysis, decision to publish, or preparation of this manuscript. The authors would like to thank Carlos José Locatelli Salgado, the veterinarian responsible for the farm where the outbreak occurred, along with the owner of the farm, for permission for sample collection and release of the results. We also thank the Department of Information Technology (Universidade Federal de Viçosa []) for supporting the data analysis on the computational cluster.

Supplementary material

705_2015_2567_MOESM1_ESM.doc (7.9 mb)
Supplementary material 1 (DOC 8099 kb)


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Rafael Locatelli Salgado
    • 1
    • 2
  • Pedro Marcus Pereira Vidigal
    • 3
  • Natalia F. Gonzaga
    • 1
    • 4
  • Luiz F. L. de Souza
    • 1
    • 4
  • Marcelo D. Polêto
    • 1
    • 2
  • Thiago Souza Onofre
    • 1
    • 2
  • Monique R. Eller
    • 5
  • Carlos Eduardo Real Pereira
    • 6
  • Juliana L. R. Fietto
    • 1
    • 2
  • Gustavo C. Bressan
    • 1
    • 2
  • Roberto M. C. Guedes
    • 6
  • Márcia R. Almeida
    • 1
    • 2
  • Abelardo Silva Júnior
    • 1
    • 4
  1. 1.Laboratório de Infectologia Molecular AnimalUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Departmento de Bioquímica e Biologia MolecularUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Núcleo de Análise de Biomoléculas (NuBiomol)Universidade Federal de ViçosaViçosaBrazil
  4. 4.Departmento de VeterináriaUniversidade Federal de ViçosaViçosaBrazil
  5. 5.Departmento de Tecnologia de AlimentosUniversidade Federal de ViçosaViçosaBrazil
  6. 6.Escola de VeterináriaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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