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Archives of Virology

, Volume 160, Issue 11, pp 2881–2885 | Cite as

Predominance of hepatitis C virus Q80K among NS3 baseline-resistance-associated amino acid variants in direct-antiviral-agent-naïve patients with chronic hepatitis: single-centre experience

  • Tina RuggieroEmail author
  • Alex Proietti
  • Lucio Boglione
  • Maria Grazia Milia
  • Tiziano Allice
  • Elisa Burdino
  • Giancarlo Orofino
  • Stefano Bonora
  • Giovanni Di Perri
  • Valeria Ghisetti
Rapid Communication

Abstract

In the era of direct-acting antiviral agents (DAAs), hepatitis C virus (HCV) genotyping tests at baseline are controversial. The HCV NS3-Q80K polymorphism is associated with resistance to the recently approved NS3 inhibitor simeprevir (SMV) when combined with PEG-interferon and ribavirin (PEG-IFN/RBV) and alternative therapy should be considered for patients with baseline Q80K. The aim of this study was to provide an estimate of Q80K prevalence at baseline in a study group of 205 DAA-naïve patients (21 % of them with HIV coinfection) using NS3 full-population direct sequencing to detect resistance-associated amino acid variants (RAVs). NS3 RAVs were identified in 56 patients (27.3 %). Q80K was the most frequently reported one (41 %), in both HIV/HCV-coinfected and HCV-monoinfected patients, but it was only detectable in cases of HCV-subtype 1a infection. Therefore, in clinical practice, an NS3-Q80K genotyping test prior to simeprevir plus PEG-IFN/RBV treatment is highly recommended.

Keywords

Sustained Virological Response Sofosbuvir Potent Antiviral Agent Baseline RAVs Baseline Q80K 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Tina Ruggiero
    • 1
    Email author
  • Alex Proietti
    • 1
  • Lucio Boglione
    • 3
  • Maria Grazia Milia
    • 1
  • Tiziano Allice
    • 1
  • Elisa Burdino
    • 1
  • Giancarlo Orofino
    • 2
  • Stefano Bonora
    • 3
  • Giovanni Di Perri
    • 3
  • Valeria Ghisetti
    • 1
  1. 1.Laboratory of Microbiology and Virology, Department of Infectious DiseasesAmedeo di Savoia HospitalTurinItaly
  2. 2.Unit A of Infectious Diseases, Department of Infectious DiseasesAmedeo di Savoia HospitalTurinItaly
  3. 3.Unit of Infectious Diseases, Department of Medical Sciences, Amedeo di Savoia HospitalUniversity of TurinTurinItaly

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