Acute human herpesvirus-6A infection of human mesothelial cells modulates HLA molecules
- 169 Downloads
Human herpesvirus 6A (HHV-6A) causes ubiquitous infections and has been associated with several diseases in immunosuppressed and immune dysregulated individuals. Although considered a lymphotropic virus, HHV-6A has the potential to infect many cell types, inducing important alterations in the infected cell. In our search for additional potential targets for HHV-6A infection, we analyzed the susceptibility of human mesothelial cells to viral infection. HHV-6A infection was performed and analyzed on primary human mesothelial cells isolated from serous cavity fluid, infected in vitro with a cell-free HHV-6A inoculum. The results demonstrated that mesothelial cells are susceptible to in vitro HHV-6A infection, and more importantly, that the virus induces an alteration of HLA expression on the cell surface, inducing HLA class II and HLA-G de novo expression. Since mesothelial cells play a pivotal role in many processes, including inflammation and antigen presentation, we speculate that, in vivo, this virus-induced perturbation might be correlated to alterations in mesothelium functions.
KeywordsHuman Leukocyte Antigen Mesothelial Cell Human Leukocyte Antigen Class Genome Equivalent Human Leukocyte Antigen Antigen
This work was supported by grants from the University of Ferrara (FAR). We thank Linda M. Sartor for revision of the English in the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Ablashi D, Agut H, Alvarez-Lafuente R, Clark DA, Dewhurst S, DiLuca D, Flamand L, Frenkel N, Gallo R, Gompels UA, Hollsberg P, Jacobson S, Luppi M, Lusso P, Malnati M, Medveczky P, Mori Y, Pellett PE, Pritchett JC, Yamanishi K, Yoshikawa T (2014) Classification of HHV-6A and HHV-6B as distinct viruses. Arch Virol 159:863–870CrossRefPubMedGoogle Scholar
- 2.Ablashi DV, Balachandran SF, Josephs Cl, Hung GRF, Kruegere B, Kramarsky SZ, Salahuddin SZ, Gallo RC (1991) Genomic polymorphism, growth properties, and immunologic variations in human herpesvirus-6 isolates. Virology 184:545–552Google Scholar
- 13.Gauster M, Blaschitz A, Dohr G (2007) Monoclonal antibody HC10 does not bind HLA-G. Rheumatol (Oxf) 46:892–893 (author reply 893–894)Google Scholar
- 21.Rotola A, Ravaioli T, Gonelli A, Dewhurst S, Cassai E, Di Luca D (1998) U94 of human herpesvirus 6 is expressed in latently infected peripheral blood mononuclear cells and blocks viral gene expression in transformed lymphocytes in culture. Proc Natl Acad Sci USA 95:13911–13916PubMedCentralCrossRefPubMedGoogle Scholar