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Complete genome sequence and phylogenetic position of the Bacillus cereus group phage JBP901

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Abstract

Bacteriophage JBP901, isolated from fermented food, is specific for Bacillus cereus group species and exhibits a broad host spectrum among a large number of B. cereus isolates. Genome sequence analysis revealed a linear 159,492-bp genome with overall G+C content of 39.7 mol%, and 201 ORFs. The presence of a putative methylase, the large number of tRNAs, and the large number of nucleotide-metabolism- and replication-related genes in JBP901 reflects its broad lytic capacity. Most of the ORFs showed a high degree of similarity to Bcp1, Bc431v3 and BCP78, and various comparative genomics analyses also consistently clustered JBP901 with orphan (unclassified) Bacillus phages in the subfamily Spounavirinae of the family Myoviridae, supporting the presence of a distinguishable group in the subfamily.

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Acknowledgments

This research was supported by High Value-Added Food Technology Development Program (project No. 112108-2), Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) and the Ministry for Food, Agriculture, Forestry, and Fisheries of Republic of Korea, and by the “Cooperative Research Program for Agriculture & Technology Development (Project No. PJ009842)”, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Korea

    Paul Tetteh Asare & Kwang-Pyo Kim

  2. Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea

    Sangryeol Ryu

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  1. Paul Tetteh Asare
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  2. Sangryeol Ryu
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  3. Kwang-Pyo Kim
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Correspondence to Kwang-Pyo Kim.

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Asare, P.T., Ryu, S. & Kim, KP. Complete genome sequence and phylogenetic position of the Bacillus cereus group phage JBP901. Arch Virol 160, 2381–2384 (2015). https://doi.org/10.1007/s00705-015-2485-0

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  • DOI: https://doi.org/10.1007/s00705-015-2485-0

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