Bufavirus in fecal specimens of patients with and without diarrhea in Thailand


Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27 %) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear.

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This study was supported by Ratchadaphisek Somphot Fund for postdoctoral fellowship; National Research Council of Thailand; The Higher Education Research Promotion and National Research University Project of Thailand Office of the Higher Education Commission (WCU-58-006-HR, WCU007-HR57, WCU 001-HR57); the Center of Excellence in Clinical Virology, Chulalongkorn University; Chulalongkorn University Centenary Academic Development Project (CU56-HR01); the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (RES560530093); the Outstanding Professor of the Thailand Research Fund (DPG5480002); the Research Chair Grant from the National Science and Technology Development Agency; MK restaurant Company Limited; The Siam Cement Group PcL; and King Chulalongkorn Memorial Hospital. We would like to thank Dr. Sompong Vongpunsawad for reviewing the manuscript.

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The authors have no conflicts of interest to declare.

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Correspondence to Yong Poovorawan.

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Chieochansin, T., Vutithanachot, V., Theamboonlers, A. et al. Bufavirus in fecal specimens of patients with and without diarrhea in Thailand. Arch Virol 160, 1781–1784 (2015).

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  • Bufavirus
  • Diarrhea
  • NS1 gene
  • Phylogenetic analysis