Archives of Virology

, Volume 160, Issue 5, pp 1325–1332 | Cite as

BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve

  • Shin-nosuke Takeshima
  • Yuri Kitamura-Muramatsu
  • Yuan Yuan
  • Meripet Polat
  • Susumu Saito
  • Yoko Aida
Brief Report

Abstract

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Because BLV infection can remain clinically silent, the proviral load is an important index for estimating disease progression. CoCoMo-qPCR, an assay developed to estimate BLV proviral load, allows the highly sensitive detection of BLV originating in different countries. Here, we developed a modified version of the CoCoMo-qPCR assay, the “BLV-CoCoMo-qPCR-2” assay, which uses optimized degenerate primers. We also constructed a new plasmid standard. Finally, we used both assays to examine DNA samples from BLV-infected cattle and compared the results.

Keywords

Bovine leukemia virus Quantitative PCR BLV-CoCoMo-qPCR Cattle CoCoMo algorithm Viral load 

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Shin-nosuke Takeshima
    • 1
    • 2
  • Yuri Kitamura-Muramatsu
    • 3
  • Yuan Yuan
    • 3
  • Meripet Polat
    • 1
    • 2
  • Susumu Saito
    • 3
  • Yoko Aida
    • 1
    • 2
  1. 1.Viral Infectious Diseases UnitRIKENWakoJapan
  2. 2.Laboratory of Viral Infectious Diseases, Department of Medical Genome Sciences, Graduate School of Frontier ScienceThe University of TokyoWakoJapan
  3. 3.Riken Yokohama InstituteRIKEN GENESIS CO., LTD.YokohamaJapan

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