Archives of Virology

, Volume 160, Issue 5, pp 1303–1311 | Cite as

Identification of novel Bromus- and Trifolium-associated circular DNA viruses

  • Simona Kraberger
  • Kata Farkas
  • Pauline Bernardo
  • Cameron Booker
  • Gerardo R. Argüello-Astorga
  • François Mesléard
  • Darren P. Martin
  • Philippe Roumagnac
  • Arvind Varsani
Brief Report


The genomes of a large number of highly diverse novel circular DNA viruses from a wide range of sources have been characterised in recent years, including circular single-stranded DNA (ssDNA) viruses that share similarities with plant-infecting ssDNA viruses of the family Geminiviridae. Here, we describe six novel circular DNA viral genomes that encode replication-associated (Rep) proteins that are most closely related to those of either geminiviruses or gemycircularviruses (a new group of ssDNA viruses that are closely related to geminiviruses). Four possible viral genomes were recovered from Bromushordeaceus sampled in New Zealand, and two were recovered from B. hordeaceus and Trifoliumresupinatum sampled in France. Two of the viral genomes from New Zealand (one from the North Island and one from the South Island each) share >99 % sequence identity, and two genomes recovered from B. hordeaceus and T. resupinatum sampled in France share 74 % identity. All of the viral genomes that were recovered were found to have a major open reading frame on both their complementary and virion-sense strands, one of which likely encodes a Rep and the other a capsid protein. Although future infectivity studies are needed to identify the host range of these viruses, this is the first report of circular DNA viruses associated with grasses in New Zealand.


Gemycircularvirus Circular DNA virus Bromus hordeaceus Trifoliumresupinatum 

Supplementary material

705_2015_2358_MOESM1_ESM.doc (73 kb)
Supplementary Table 1 Details of grass samples collected and screened for this study (DOC 73 kb)
705_2015_2358_MOESM2_ESM.doc (32 kb)
Supplementary Table 2 Details for bromus-associated circular DNA viral isolates and back-to-back primers used to recover full genomes (DOC 32 kb)
705_2015_2358_MOESM3_ESM.pdf (406 kb)
Supplementary Figure 1 Comparisons of the replication origin regions of BasCV-3 and SsHADV-1, illustrating the resemblance between their putative Rep-binding sites (iterons). Note that the nick site (the conserved nonanucleotide) of BasCV-3 differs from that of SsHADV-1 at position N5 (PDF 406 kb)
705_2015_2358_MOESM4_ESM.pdf (431 kb)
Supplementary Figure 2 Correlations between iteron core sequences and potential Rep DNA-binding SPDs of BasCV-3 and selected gemycircularviruses. Amino acid residues identified as putative SPDs in the beta-1 strand (r1) are shaded in yellow, whereas SPDs in the beta-strand (r-2) associated with motif II are shaded in blue. The conserved RCR motifs I and II are indicated at the top of the alignments. The N-terminus of the Rep of BasCV-1 is also aligned to show the similarity of its RCR motifs with those of gemycircularviruses (PDF 431 kb)


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Simona Kraberger
    • 1
  • Kata Farkas
    • 1
  • Pauline Bernardo
    • 2
  • Cameron Booker
    • 3
  • Gerardo R. Argüello-Astorga
    • 4
  • François Mesléard
    • 5
    • 6
  • Darren P. Martin
    • 7
  • Philippe Roumagnac
    • 2
  • Arvind Varsani
    • 1
    • 8
    • 9
  1. 1.School of Biological Sciences and Biomolecular Interaction CentreUniversity of CanterburyChristchurchNew Zealand
  2. 2.CIRAD-INRA-SupAgro, UMR BGPI, Campus International de Montferrier-BaillarguetMontpellier Cedex-5France
  3. 3.RangiroaNew Zealand
  4. 4.División de Biología MolecularInstituto Potosino de Investigación Científica y TecnológicaSan Luis PotosíMexico
  5. 5.Université d’Avignon et des Pays de Vaucluse, UMR CNRS/IRD IMBE 7263/237, Institut Méditerranéen de Biodiversité et d’Écologie, IUT AgroparcBP 61207 Avignon Cedex 9France
  6. 6.Tour du Valat, Centre de recherche pour la conservation des zones humides méditerranéennes13 200 Le Sambuc-ArlesFrance
  7. 7.Computational Biology Group, Institute of Infectious Diseases and Molecular MedicineUniversity of Cape TownCape TownSouth Africa
  8. 8.Electron Microscope Unit, Division of Medical Biochemistry, Department of Clinical Laboratory SciencesUniversity of Cape TownCape TownSouth Africa
  9. 9.Department of Plant Pathology and Emerging Pathogens InstituteUniversity of FloridaGainesvilleUSA

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