Complete genome sequence of yacon necrotic mottle virus, a novel putative member of the genus Badnavirus
- 453 Downloads
The complete genome sequence of a previously undescribed virus isolated from a yacon plant exhibiting necrotic mottle, chlorosis, stunting, and leaf malformation symptoms in Gyeongju, Korea, was determined. The genome of this virus consists of one circular double-stranded DNA of 7661 bp in size. The genome contained four open reading frames (ORFs 1 to 4) on the plus strand that potentially encode proteins of 26, 32, 234, and 25 kDa. Protein BLAST analysis showed that ORF3, which is the largest ORF, has 45 % amino acid sequence identity (with 89 % coverage) to the ORF3 of fig badnavirus 1 (FBV-1), a recently identified badnavirus. Phylogenetic analysis provided further evidence that the virus identified in this study is probably a member of a new species in the genus Badnavirus. The name yacon necrotic mottle virus (YNMoV) is proposed for this new virus.
KeywordsAmino Acid Sequence Identity Circular Genome Putative ORFs Putative Member High Amino Acid Sequence Identity
This research was supported by a grant from the Agenda Program (PJ008841), funded by the Rural Development Administration of Korea.
- 4.Geering ADW, Hull R (2012) Family Caulimoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier, LondonGoogle Scholar
- 10.Seal S, Turaki A, Muller E, Kumar PL, Kenyon L, Filloux D, Galzi S, Lopez-Montes A, Iskra-Caruana ML (2014) The prevalence of badnaviruses in West African yams (Dioscorea cayenensis-rotundata) and evidence of endogenous pararetrovirus sequences in their genomes. Virus Res 186:144–154CrossRefPubMedGoogle Scholar