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Archives of Virology

, Volume 160, Issue 3, pp 873–875 | Cite as

Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana

  • Ioly Kotta-LoizouEmail author
  • Jana Sipkova
  • Robert H. A. Coutts
Annotated Sequence Record

Abstract

An isolate of the entomopathogenic fungus Beauveria bassiana was found to contain five double-stranded (ds) RNA elements ranging from 1.5 to more than 3 kbp. The complete sequence of the largest dsRNA element is described here. Analysis of the RdRp nucleotide sequence reveals its similarity to unclassified dsRNA elements, such as Alternaria longipes dsRNA virus 1, and its distant relationship to the RNA-dependent RNA polymerases of members of the family Partitiviridae.

Keywords

Graminearum dsRNA Virus Fungus Beauveria Bassiana dsRNA Element Number LN610699 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank Professor Milton A. Typas for providing us with the B. bassiana isolate EABb 92/11-Dm from a fungal collection kept in the Department of Genetics and Biotechnology, University of Athens, Greece.

References

  1. 1.
    Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefPubMedCentralPubMedGoogle Scholar
  2. 2.
    Banks GT, Buck KW, Chain EB, Darbyshire JE, Himmelweit F (1969) Virus-like particles in penicillin producing strains of Penicillium chrysogenum. Nature 223:89–90CrossRefGoogle Scholar
  3. 3.
    Coutts RHA, Livieratos IC (2003) A rapid method for sequencing the 5′- and 3′-termini of dsRNA viral templates using RLM-RACE. J Phytopathol 151:525–527CrossRefGoogle Scholar
  4. 4.
    Froussard P (1992) A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA. Nucleic Acids Res 20:2900CrossRefPubMedCentralPubMedGoogle Scholar
  5. 5.
    Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384CrossRefPubMedGoogle Scholar
  6. 6.
    Ghikas DV, Kouvelis VN, Typas MA (2010) Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii. BMC Microbiol 10:174CrossRefPubMedCentralPubMedGoogle Scholar
  7. 7.
    Kwon S-J, Lim W-S, Park S-H, Park M-R, Kim K-H (2009) Molecular characterization of a dsRNA mycovirus, Fusarium graminearum virus-DK21, which is phylogenetically related to hypoviruses but has a genome organization and gene expression strategy resembling those of plant potex-like viruses. Mol Cells 28:73–74CrossRefGoogle Scholar
  8. 8.
    Herrero N, Duenas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530CrossRefPubMedCentralPubMedGoogle Scholar
  9. 9.
    Lin Y, Zhang H, Zhao C, Liu S, Guo L (2014) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol. doi: 10.1007/s00705-014-2218-9 PubMedCentralGoogle Scholar
  10. 10.
    Liu H, Fu Y, Jiang D, Li G, Xie J, Peng Y, Yi X, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen hepatitis E virus and rubi-like viruses. J Virol 83:1981–1991CrossRefPubMedCentralPubMedGoogle Scholar
  11. 11.
    Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642CrossRefPubMedGoogle Scholar
  12. 12.
    Yie SW, Khalifa ME, Hahn T, Pearson MN (2014) Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 159:1321–1327CrossRefPubMedGoogle Scholar
  13. 13.
    Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392CrossRefPubMedCentralPubMedGoogle Scholar
  14. 14.
    Zhang T, Jiang Y, Dong W (2014) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology. doi: 10.1016/j.virol.2014.06.003 Google Scholar
  15. 15.
    Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415CrossRefPubMedCentralPubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Ioly Kotta-Loizou
    • 1
    Email author
  • Jana Sipkova
    • 1
  • Robert H. A. Coutts
    • 1
  1. 1.Department of Life Sciences, Faculty of Natural SciencesImperial College LondonLondonUK

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