Complete genome sequences of two biologically distinct isolates of Asparagus virus 1
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The complete genome sequences of two asparagus virus 1 (AV-1) isolates differing in their ability to cause systemic infection in Nicotiana benthamiana were determined. Their genomes had 9,741 nucleotides excluding the 3′-terminal poly(A) tail, encoded a polyprotein of 3,112 amino acids, and shared 99.6 % nucleotide sequence identity. They differed at 37 nucleotide and 15 amino acid sequence positions (99.5 % identity) scattered over the polyprotein. The closest relatives of AV-1 in amino acid sequence identity were plum pox virus (54 %) and turnip mosaic virus (53 %), corroborating the classification of AV-1 as a member of a distinct species in the genus Potyvirus.
KeywordsQuery Coverage Coat Protein Sequence Turnip Mosaic Virus Asparagus Virus Partial Coat Protein
We thank Dr. Wulf Menzel (German Collection of Microorganisms and Cell Cultures DSMZ, Braunschweig, Germany) for providing the isolate samples, Dr. Frank Rabenstein (Julius Kühn-Institut, Quedlinburg, Germany) for providing purified virus material of AV-1, and Dr. Lawrence Kenyon (AVRDC - The World Vegetable Center, Shanhua, Taiwan) for his suggestions to improve the writing and language style.
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