Archives of Virology

, Volume 160, Issue 2, pp 577–580 | Cite as

The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28

  • Yanhong Lin
  • Hailong Zhang
  • Chengjin Zhao
  • Shengxue LiuEmail author
  • Lihua GuoEmail author
Annotated Sequence Record


The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3′ poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5′-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3′-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.


Strain HN28 dsRNA Virus Brown Spot Disease dsRNA Genome Segment Cryphonectria Hypovirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the National Natural Science Foundation of China (31171818). We greatly thank Dr. Meng Zhang at Henan Agricultural University, Zhengzhou, Henan, China, for assistance with fungal identification.


  1. 1.
    Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140:179–187PubMedCrossRefGoogle Scholar
  2. 2.
    Howitt RLJ, Beever RE, Pearson MN, Forster RLS (2006) Genome characterization of a flexuous rod-shaped mycovirus, Botrytis virus X, reveals high amino acid identity to genes from plant ‘potex-like’viruses. Arch Virol 151:563–579PubMedCrossRefGoogle Scholar
  3. 3.
    King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) (2011) Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, p 1248Google Scholar
  4. 4.
    Liu HQ, Fu YP, Jiang DH, Li GQ, Xie J, Peng YL, XH Y, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen hepatitis E virus and rubi-like viruses. J Virol 83:1981–1991PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Luo YY, Yang JK, Zhu ML, Liu CJ, Li HY, Lu ZB, Pan WZ, Zhang ZH, Bi W, Zhang KQ (2012) The group III two-component histidine kinase AlHK1 is involved in fungicides resistance, osmosensitivity, spore production and impacts negatively pathogenicity in Alternaria longipes. Curr Microbiol 64:449–456PubMedCrossRefGoogle Scholar
  6. 6.
    Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642PubMedCrossRefGoogle Scholar
  7. 7.
    Shepherd HS (1988) Virus-like particles in tentoxin-production strains of Alternaria alternata. J Virol 62:3888–3891PubMedCentralPubMedGoogle Scholar
  8. 8.
    Suzuki NS, Supyani K, Maruyama, Hillman BI (2004) Complete genome sequence of Mycoreovirus 1/Cp9B21, a member of a new genus within the family Reoviridae, from the chestnut blight fungus Cryphonectria parasitica. J Gen Virol 85:3437–3448PubMedCrossRefGoogle Scholar
  9. 9.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    Valverde RA, Nameth ST, JORDAN RL (1990) Analysis of double-stranded RNA for plant virus diagnosis. Plant Dis 74:255–258CrossRefGoogle Scholar
  11. 11.
    Vainio EJ, Hyder R, Aday G, Hansen E, Piri T, Doğmuş-Lehtijärvi T, Lehtijärvi A, Korhonen K, Hantula J (2012) Population structure of a novel putative mycovirus infecting the conifer root-rot fungus Heterobasidion annosum sensu lato. Virology 422:366–376PubMedCrossRefGoogle Scholar
  12. 12.
    Wang S, Kondo H, Liu L, Guo L, Qiu D (2013) A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. Virus Res 174:69–77PubMedCrossRefGoogle Scholar
  13. 13.
    Woudenberg JHC, Groenewald JZ, Binder M, Crous PW (2013) Alternaria redefined. Stud Mycol 75:171–212PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    Nibert ML, Ghabrial SA, Maiss E, Lesker T (eds) (2013) A taxonomic proposal of the International Committee on Taxonomy of Viruses: Reorganization of family Partitiviridae to contain 4 new genera of plant and/or fungal viruses, with elimination of 3 current genera and creation of 16 new speciesGoogle Scholar
  15. 15.
    Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci 107:8387–8392PubMedCentralPubMedCrossRefGoogle Scholar
  16. 16.
    Yu J, Kwon SJ, Lee KM, Son M, Kim KH (2009) Complete nucleotide sequence of double-stranded RNA viruses from Fusarium graminearum strain DK3. Arch Virol 154:1855–1858PubMedCrossRefGoogle Scholar
  17. 17.
    Zheng L, Liu H, Zhang M, Cao X, Zhou E (2013) The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275. Arch Virol 158:1609–1612PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  1. 1.Key Laboratory at Universities of Xinjiang, Uygur Autonomous Region for Oasis, Agricultural Pest Management and Plant Protection Resource UtilizationShihezi UniversityShiheziChina
  2. 2.The Institute of Plant Protection/State Key Laboratory for Biology of Plant Diseases and Insect PestsChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

Personalised recommendations