Characterization and genome sequence of Dev2, a new T7-like bacteriophage infecting Cronobacter turicensis
- 477 Downloads
Cronobacter spp. are opportunistic pathogenic bacteria that are responsible for severe infections in neonates. Powdered infant formula was confirmed to be the source in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study, we investigated the growth parameters and genome organization of a new bacteriophage, Dev2, isolated from sewage. The Dev2 phage contains DNA with a length of 39 kb and belongs to the T7 branch of the subfamily Autographivirinae, with the highest degree of identity to the phage K1F. The host specificity of Dev2 is limited to C. turicensis strains of the CT O:1 serotype. With a lower efficiency, this phage also infects some Salmonella and E. coli strains. The Dev2 phage can inactivate sensitive Cronobacter strains in reconstituted milk formula. The results obtained in this study are an important prerequisite for application of Dev2 in food control.
KeywordsPhage Particle Phage Therapy Powdered Infant Formula Overnight Bacterial Culture Tail Fiber Protein
This publication is a result of implementation the project REVOGENE – Research Centre for Molecular Genetics (ITMS 26240220067), supported by the Research & Development Operational Programme funded by the ERDF. This work was supported by the Slovak Research and Development Agency under contract no. APVV-0098-10.
- 4.Abbasifar R, Kropinski AM, Sabour PM, Chambers JR, Mackinnon J, Malig T, Griffiths MW (2014) Efficiency of bacteriophage therapy against Cronobacter sakazakii in Galleria mellonella (greater wax moth) larvae. Arch Virol. doi: 10.1007/s00705-014-2055-x
- 5.Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O (2008) The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75PubMedCrossRefPubMedCentralGoogle Scholar
- 8.Clokie MRJ, Kropinski AM (eds) (2009) Bacteriophages: methods and protocols, vol 1: isolation, characterization, and interactions, vol 501. Humana Press, New YorkGoogle Scholar
- 11.Fao/WHO (2008) Enterobacter sakazakii (Cronobacter spp.) in powdered follow-up formulae: meeting report. Microbiological risk assessment series no 15, Rome, Italy, p 90Google Scholar
- 16.Jarvis KG, Grim CJ, Franco AA, Gopinath G, Sathyamoorthy V, Hu L, Sadowski JA, Lee CS, Tall BD (2011) Molecular characterization of Cronobacter lipopolysaccharide O-antigen gene clusters and development of serotype-specific PCR assays. Appl Environ Microbiol 77:4017–4026PubMedCrossRefPubMedCentralGoogle Scholar
- 30.Sun Y, Wang M, Wang Q, Cao B, He X, Li K, Feng L, Wang L (2012) Genetic analysis of the Cronobacter sakazakii O4 to O7 O-antigen gene clusters and development of a PCR assay for identification of all C. sakazakii O serotypes. Appl Environ Microbiol 78:3966–3974PubMedCrossRefPubMedCentralGoogle Scholar