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Archives of Virology

, Volume 159, Issue 11, pp 3013–3019 | Cite as

Characterization and genome sequence of Dev2, a new T7-like bacteriophage infecting Cronobacter turicensis

  • Michal Kajsík
  • Lucia Oslanecová
  • Tomáš Szemes
  • Michalea Hýblová
  • Andrea Bilková
  • Hana DrahovskáEmail author
  • Ján Turňa
Original Article

Abstract

Cronobacter spp. are opportunistic pathogenic bacteria that are responsible for severe infections in neonates. Powdered infant formula was confirmed to be the source in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study, we investigated the growth parameters and genome organization of a new bacteriophage, Dev2, isolated from sewage. The Dev2 phage contains DNA with a length of 39 kb and belongs to the T7 branch of the subfamily Autographivirinae, with the highest degree of identity to the phage K1F. The host specificity of Dev2 is limited to C. turicensis strains of the CT O:1 serotype. With a lower efficiency, this phage also infects some Salmonella and E. coli strains. The Dev2 phage can inactivate sensitive Cronobacter strains in reconstituted milk formula. The results obtained in this study are an important prerequisite for application of Dev2 in food control.

Keywords

Phage Particle Phage Therapy Powdered Infant Formula Overnight Bacterial Culture Tail Fiber Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This publication is a result of implementation the project REVOGENE – Research Centre for Molecular Genetics (ITMS 26240220067), supported by the Research & Development Operational Programme funded by the ERDF. This work was supported by the Slovak Research and Development Agency under contract no. APVV-0098-10.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Michal Kajsík
    • 1
  • Lucia Oslanecová
    • 1
  • Tomáš Szemes
    • 3
  • Michalea Hýblová
    • 1
  • Andrea Bilková
    • 2
  • Hana Drahovská
    • 1
    Email author
  • Ján Turňa
    • 1
  1. 1.Department of Molecular Biology, Faculty of Natural SciencesComenius UniversityBratislavaSlovakia
  2. 2.Faculty of PharmacyComenius UniversityBratislavaSlovakia
  3. 3.Geneton Ltd.BratislavaSlovakia

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