Archives of Virology

, Volume 159, Issue 11, pp 3157–3160 | Cite as

Molecular characterisation of novel mitoviruses associated with Sclerotinia sclerotiorum

  • Mahmoud E. Khalifa
  • Michael N. Pearson
Annotated Sequence Record


Seven putative mitoviral genomes, representing four species from three Sclerotinia sclerotiorum isolates, were fully sequenced. The genome lengths ranged from 2438 to 2815 nucleotides. The RNA-dependent RNA polymerase (RdRp) of one genome shared high amino acid (aa) sequence identity (98.5 %) with the previously described Sclerotinia sclerotiorum mitovirus 2 (SsMV2/NZ1) and was provisionally assigned the name SsMV2/14563. The RdRps of three of the genomes with closest aa sequence identity of 78.8-79.3 % to Sclerotinia sclerotiorum mitovirus 1 (SsMV1/KL1) were provisionally considered to represent a new species, and the corresponding virus was named Sclerotinia sclerotiorum mitovirus 5 (SsMV5/11691, SsMV5/14563 and SsMV5/Lu471). The remaining two novel genomes, for which the viruses were provisionally named Sclerotinia sclerotiorum mitovirus 6 (SsMV6/14563 and SsMV6/Lu471) and Sclerotinia sclerotiorum mitovirus 7 (SsMV7/Lu471), showed closest aa sequence identities to Sclerotinia sclerotiorum mitovirus 3 (SsMV3/NZ1; 57.5-57.8 %) and Cryphonectria cubensis mitovirus 1a (CcMV1a; 32 %), respectively. The RdRp proteins of all seven genomes contained the conserved aa sequence motifs (I-IV) previously reported for mitoviruses, and their 5′ and 3′ untranslated regions (UTRs) have the potential to fold into stem-loop secondary structures.


Sclerotinia Sclerotiorum dsRNA Segment Hairpin Secondary Structure Total Nucleic Acid Extract Chatham Island 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was funded by The Bio-protection Research Centre and The University of Auckland, New Zealand. We wish to thank the two anonymous reviewers for valuable comments.

Supplementary material

705_2014_2171_MOESM1_ESM.pdf (196 kb)
Supplementary material 1 (PDF 195 kb)
705_2014_2171_MOESM2_ESM.pdf (52 kb)
Supplementary material 2 (PDF 51 kb)


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  1. 1.School of Biological SciencesThe University of AucklandAucklandNew Zealand

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