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Archives of Virology

, Volume 159, Issue 11, pp 3157–3160 | Cite as

Molecular characterisation of novel mitoviruses associated with Sclerotinia sclerotiorum

  • Mahmoud E. Khalifa
  • Michael N. Pearson
Annotated Sequence Record

Abstract

Seven putative mitoviral genomes, representing four species from three Sclerotinia sclerotiorum isolates, were fully sequenced. The genome lengths ranged from 2438 to 2815 nucleotides. The RNA-dependent RNA polymerase (RdRp) of one genome shared high amino acid (aa) sequence identity (98.5 %) with the previously described Sclerotinia sclerotiorum mitovirus 2 (SsMV2/NZ1) and was provisionally assigned the name SsMV2/14563. The RdRps of three of the genomes with closest aa sequence identity of 78.8-79.3 % to Sclerotinia sclerotiorum mitovirus 1 (SsMV1/KL1) were provisionally considered to represent a new species, and the corresponding virus was named Sclerotinia sclerotiorum mitovirus 5 (SsMV5/11691, SsMV5/14563 and SsMV5/Lu471). The remaining two novel genomes, for which the viruses were provisionally named Sclerotinia sclerotiorum mitovirus 6 (SsMV6/14563 and SsMV6/Lu471) and Sclerotinia sclerotiorum mitovirus 7 (SsMV7/Lu471), showed closest aa sequence identities to Sclerotinia sclerotiorum mitovirus 3 (SsMV3/NZ1; 57.5-57.8 %) and Cryphonectria cubensis mitovirus 1a (CcMV1a; 32 %), respectively. The RdRp proteins of all seven genomes contained the conserved aa sequence motifs (I-IV) previously reported for mitoviruses, and their 5′ and 3′ untranslated regions (UTRs) have the potential to fold into stem-loop secondary structures.

Keywords

Sclerotinia Sclerotiorum dsRNA Segment Hairpin Secondary Structure Total Nucleic Acid Extract Chatham Island 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was funded by The Bio-protection Research Centre and The University of Auckland, New Zealand. We wish to thank the two anonymous reviewers for valuable comments.

Supplementary material

705_2014_2171_MOESM1_ESM.pdf (196 kb)
Supplementary material 1 (PDF 195 kb)
705_2014_2171_MOESM2_ESM.pdf (52 kb)
Supplementary material 2 (PDF 51 kb)

References

  1. 1.
    Boland GJ, Hall R (1994) Index of plant hosts of Sclerotinia sclerotiorum. Can J Plant Pathol 16:93–108CrossRefGoogle Scholar
  2. 2.
    Cole TE, Hong Y, Brasier CM, Buck KW (2000) Detection of an RNA-dependent RNA polymerase in mitochondria from a mitovirus-infected isolate of the Dutch Elm disease fungus, Ophiostoma novo-ulmi. Virology 268:239–243PubMedCrossRefGoogle Scholar
  3. 3.
    Hillman BI, Esteban R (2012) Family-Narnaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Elsevier, San Diego, pp 1025–1030Google Scholar
  4. 4.
    Hintz WE, Carneiro JS, Kassatenko I, Varga A, James D (2013) Two novel mitoviruses from a Canadian isolate of the Dutch elm pathogen Ophiostoma novo-ulmi (93-1224). Virol J 10:252PubMedCrossRefPubMedCentralGoogle Scholar
  5. 5.
    Hong Y, Cole TE, Brasier CM, Buck KW (1998) Evolutionary relationships among putative RNA-dependent RNA polymerases encoded by a mitochondrial virus-like RNA in the Dutch elm disease fungus, Ophiostoma novo-ulmi, by other viruses and virus-like RNAs and by the Arabidopsis mitochondrial genome. Virology 246:158–169PubMedCrossRefGoogle Scholar
  6. 6.
    Hong Y, Cole TE, Brasier CM, Buck KW (1998) Novel structures of two virus-like RNA elements from a diseased isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi. Virology 242:80–89PubMedCrossRefGoogle Scholar
  7. 7.
    Hong Y, Dover SL, Cole TE, Brasier CM, Buck KW (1999) Multiple mitochondrial viruses in an isolate of the Dutch elm disease fungus Ophiostoma novo-ulmi. Virology 258:118–127PubMedCrossRefGoogle Scholar
  8. 8.
    Howitt RLJ, Beever RE, Pearson MN, Forster RLS (1995) Presence of double-stranded RNA and virus-like particles in Botrytis cinerea. Mycol Res 99:1472–1478CrossRefGoogle Scholar
  9. 9.
    Huelsenbeck JP (1995) The robustness of two phylogenetic methods: four-taxon simulations reveal a slight superiority of maximum likelihood over neighbor joining. Mol Biol Evol 12:843–849PubMedGoogle Scholar
  10. 10.
    Khalifa ME, Pearson MN (2013) Molecular characterization of three mitoviruses co-infecting a hypovirulent isolate of Sclerotinia sclerotiorum fungus. Virology 441:22–30PubMedCrossRefGoogle Scholar
  11. 11.
    Mathews DH, Sabina J, Zuker M, Turner DH (1999) Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. J Mol Biol 288:911–940PubMedCrossRefGoogle Scholar
  12. 12.
    Osaki H, Nakamura H, Nomura K, Matsumoto N, Yoshida K (2005) Nucleotide sequence of a mitochondrial RNA virus from the plant pathogenic fungus, Helicobasidium mompa Tanaka. Virus Res 107:39–46PubMedCrossRefGoogle Scholar
  13. 13.
    Park Y, Chen X, Punja ZK (2006) Molecular and biological characterization of a mitovirus in Chalara elegans (Thielaviopsis basicola). Phytopathology 96:468–479PubMedCrossRefGoogle Scholar
  14. 14.
    Valverde RA, Nameth ST, Jordan RL (1990) Analysis of double-stranded RNA for plant virus diagnosis. Plant Dis 74:255–258CrossRefGoogle Scholar
  15. 15.
    Xie J, Ghabrial SA (2012) Molecular characterization of two mitoviruses co-infecting a hypovirulent isolate of the plant pathogenic fungus Sclerotinia sclerotiorum. Virology 428:77–85PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  1. 1.School of Biological SciencesThe University of AucklandAucklandNew Zealand

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