The complete nucleotide sequence of a novel partitivirus isolated from the plant pathogenic fungus Verticillium albo-atrum
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We have characterized the bisegmented genome of a novel double-stranded RNA (dsRNA) virus isolated from the plant pathogenic fungus Verticillium albo-atrum. We determined that its larger segment (dsRNA1) was 1747 base pairs in length and potentially encoded an RNA-dependent RNA polymerase of 539 amino acids, whereas the smaller segment (dsRNA2) was 1517 base pairs long and was predicted to encode a capsid protein of 435 amino acids. Homology searches and phylogenetic analysis confirmed that, as expected from its dsRNA banding profile, the identified virus was a new member of the family Partitiviridae, and we have therefore designated it V. a lbo- a trum partitivirus 1 (VaaPV1). This is the first report of a mycovirus identified in V. albo-atrum.
KeywordsVerticillium Wilt Ascomycetous Fungus AAFC dsRNA Segment Asexual Sporulation
This work was supported by the Spanish Ministry of Science and Innovation Grant No. AGL2009-13445 awarded to M.D.G.-P. We thank K.F.D. and AAFC for the V. albo-atrum strain used in this study.
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