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Archives of Virology

, Volume 159, Issue 11, pp 2937–2948 | Cite as

Toll-like receptor 3 inhibits Newcastle disease virus replication through activation of pro-inflammatory cytokines and the type-1 interferon pathway

  • Jinghua Cheng
  • Yingjie Sun
  • Xiaorong Zhang
  • Fanqing Zhang
  • Shilei Zhang
  • Shengqing Yu
  • Xusheng Qiu
  • Lei Tan
  • Cuiping Song
  • Song Gao
  • Yantao WuEmail author
  • Chan DingEmail author
Original Article

Abstract

Newcastle disease virus (NDV) is an avian paramyxovirus that can selectively replicate in and destroy human tumor cells. In this report, we demonstrate that NDV infection in HeLa cells leads to the activation of the pattern recognition Toll-like receptor 3 (TLR3). Overexpression of TLR3 enhanced the activity of the IFN-β promoter and the transcription factor NF-kappa B (NF-κB), thereby decreasing viral protein synthesis and the virus titer. In addition, the reduction of endogenous TLR3 by small interfering RNA (siRNA) increased NDV replication. Similar anti-NDV effects were observed in DF-1 chicken fibroblast cells with the exogenous expression of chicken TLR3 (cTLR3). Immunofluorescence staining of HeLa cells indicated that the dsRNA generated during NDV replication colocalized with TLR3 in punctate subcellular structures. Altogether, our results strongly suggest that TLR3 actively participates in the recognition of the innate pro-inflammatory response after NDV infection and leads to the consequent antiviral cytokine/interferon secretion.

Keywords

Respiratory Syncytial Virus West Nile Virus Newcastle Disease Virus TLR3 Expression Newcastle Disease Virus Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to Professor Takashi Fujita (Kyoto University, Japan) for providing the p125-luc plasmid. This work was financially supported by the Chinese National High-Tech R&D Program (2011AA10A209), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the China Agriculture Research System (CARS-41-K08), and the Chinese Special Fund for Agro-Scientific Research in the Public Interest (201003012 and 201303033).

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Jinghua Cheng
    • 1
    • 2
  • Yingjie Sun
    • 2
  • Xiaorong Zhang
    • 1
  • Fanqing Zhang
    • 3
  • Shilei Zhang
    • 2
  • Shengqing Yu
    • 2
  • Xusheng Qiu
    • 2
  • Lei Tan
    • 2
  • Cuiping Song
    • 2
  • Song Gao
    • 1
  • Yantao Wu
    • 1
    Email author
  • Chan Ding
    • 2
    Email author
  1. 1.Jiangsu Co-Innovation Center for Prevention and Control of Animal Infectious Diseases and ZoonosesCollege of Veterinary Medicine, Yangzhou UniversityYangzhouPeople’s Republic of China
  2. 2.Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghaiPeople’s Republic of China
  3. 3.College of Veterinary MedicineNanjing Agriculture UniversityNanjingPeople’s Republic of China

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