Archives of Virology

, Volume 159, Issue 10, pp 2693–2697 | Cite as

Comparison of the copy numbers of bovine leukemia virus in the lymph nodes of cattle with enzootic bovine leukosis and cattle with latent infection

  • Yoshiko Somura
  • Emi Sugiyama
  • Hiroshi Fujikawa
  • Kenji Murakami
Original Article


To establish a diagnostic index for predicting enzootic bovine leukosis (EBL), proviral bovine leukemia virus (BLV) copies in whole blood, lymph nodes and spleen were examined by quantitative real-time PCR (qPCR). Cattle were divided into two groups, EBL and BLV-infected, based on meat inspection data. The number of BLV copies in all specimens of EBL cattle was significantly higher than those of BLV-infected cattle (p < 0.0001), and the number of BLV copies in the lymph nodes was particularly large. Over 70 % of the superficial cervical, medial iliac and jejunal lymph nodes from EBL cattle had more than 1,000 copies/10 ng DNA, whereas lymph nodes from BLV-infected cattle did not. These findings suggest that the cattle harboring more than 1,000 BLV copies may be diagnosed with EBL.


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Yoshiko Somura
    • 1
    • 2
  • Emi Sugiyama
    • 3
  • Hiroshi Fujikawa
    • 2
    • 4
  • Kenji Murakami
    • 2
    • 5
  1. 1.Department of MicrobiologyTokyo Metropolitan Institute of Public HealthShinjukuJapan
  2. 2.The United Graduate School of Veterinary SciencesGifu UniversityGifuJapan
  3. 3.Tokyo Metropolitan Wholesale Market Sanitation Inspection StationChuoJapan
  4. 4.Cooperative Department of Veterinary Medicine, Faculty of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan
  5. 5.Cooperative Department of Veterinary Medicine, Faculty of AgricultureIwate UniversityMoriokaJapan

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