Archives of Virology

, Volume 159, Issue 10, pp 2549–2557 | Cite as

Peroxisome-localized hepatitis Bx protein increases the invasion property of hepatocellular carcinoma cells

  • Jae-Min Han
  • Jung-Ah Kang
  • Min-Hee Han
  • Kyung-Hun Chung
  • Cho-Rong Lee
  • Woo-Keun Song
  • Youngsoo Jun
  • Sung-Gyoo Park
Original Article


HBx acts as a multifunctional regulator that modulates various cellular responses, which can lead to development and progression of hepatocellular carcinoma (HCC). Here, we show that the HBx protein is also localized to peroxisomes, and this increases cellular reactive oxygen species (ROS) to levels that are higher than when HBx is localized to other organelles. The elevated ROS strongly activated nuclear factor (NF)-κB. In addition, the peroxisome-localized HBx increased the expressions of matrix metalloproteinases and decreased the expression of E-cadherin, which increased the invasive ability of HCC cells. Thus, a specific distribution of HBx to peroxisomes may contribute to HCC progression by increasing the invasive ability of HCC cells through elevation of the cellular ROS level.


Green Fluorescent Protein HepG2 Cell Reactive Oxygen Species Level Hep3B Cell Quantitative Reverse Transcription Polymerase Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Hepatitis B virus


Hepatocellular carcinoma


Activation protein-1


Nuclear factor κB


Reactive oxygen species


Matrix metalloproteinases




Mitochondrial fission 1 protein


Monoclonal antibody


Polyclonal antibody


Dulbecco’s modified Eagle’s medium


Fetal bovine serum


Sodium dodecyl sulfate


Phosphate-buffered saline


Internal ribosome entry site


Green fluorescent protein


Reverse transcription polymerase chain reaction


Glyceraldehyde-3-phosphate dehydrogenase


Mitochondrial antiviral signaling


N-Acetyl cysteine


Peroxisomal biogenesis factor 5



This work was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A111838) and by the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (MEST)(NRF-2013R1A1A2010995).

Conflict of interest

We confirm that all authors fulfill all conditions required for authorship. We also confirm that there is no potential conflict of interest or financial dependence regarding this publication, as described in the Instructions for Authors. All authors have read and approved the manuscript.


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Jae-Min Han
    • 1
    • 2
  • Jung-Ah Kang
    • 1
    • 2
  • Min-Hee Han
    • 1
    • 2
  • Kyung-Hun Chung
    • 1
  • Cho-Rong Lee
    • 1
    • 2
  • Woo-Keun Song
    • 1
  • Youngsoo Jun
    • 1
    • 2
  • Sung-Gyoo Park
    • 1
    • 2
  1. 1.School of Life SciencesGwangju Institute of Science and Technology (GIST)GwangjuRepublic of Korea
  2. 2.Immune Synapse Research CenterGwangju Institute of Science and Technology (GIST)GwangjuRepublic of Korea

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