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Archives of Virology

, Volume 159, Issue 10, pp 2549–2557 | Cite as

Peroxisome-localized hepatitis Bx protein increases the invasion property of hepatocellular carcinoma cells

  • Jae-Min Han
  • Jung-Ah Kang
  • Min-Hee Han
  • Kyung-Hun Chung
  • Cho-Rong Lee
  • Woo-Keun Song
  • Youngsoo Jun
  • Sung-Gyoo Park
Original Article

Abstract

HBx acts as a multifunctional regulator that modulates various cellular responses, which can lead to development and progression of hepatocellular carcinoma (HCC). Here, we show that the HBx protein is also localized to peroxisomes, and this increases cellular reactive oxygen species (ROS) to levels that are higher than when HBx is localized to other organelles. The elevated ROS strongly activated nuclear factor (NF)-κB. In addition, the peroxisome-localized HBx increased the expressions of matrix metalloproteinases and decreased the expression of E-cadherin, which increased the invasive ability of HCC cells. Thus, a specific distribution of HBx to peroxisomes may contribute to HCC progression by increasing the invasive ability of HCC cells through elevation of the cellular ROS level.

Keywords

Green Fluorescent Protein HepG2 Cell Reactive Oxygen Species Level Hep3B Cell Quantitative Reverse Transcription Polymerase Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HBV

Hepatitis B virus

HCC

Hepatocellular carcinoma

AP-1

Activation protein-1

NF-κB

Nuclear factor κB

ROS

Reactive oxygen species

MMPs

Matrix metalloproteinases

HA

Hemagglutinin

FIS1

Mitochondrial fission 1 protein

mAb

Monoclonal antibody

pAb

Polyclonal antibody

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

SDS

Sodium dodecyl sulfate

PBS

Phosphate-buffered saline

IRES

Internal ribosome entry site

GFP

Green fluorescent protein

RT-PCR

Reverse transcription polymerase chain reaction

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

MAVS

Mitochondrial antiviral signaling

NAC

N-Acetyl cysteine

PEX5

Peroxisomal biogenesis factor 5

Notes

Acknowledgments

This work was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A111838) and by the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (MEST)(NRF-2013R1A1A2010995).

Conflict of interest

We confirm that all authors fulfill all conditions required for authorship. We also confirm that there is no potential conflict of interest or financial dependence regarding this publication, as described in the Instructions for Authors. All authors have read and approved the manuscript.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Jae-Min Han
    • 1
    • 2
  • Jung-Ah Kang
    • 1
    • 2
  • Min-Hee Han
    • 1
    • 2
  • Kyung-Hun Chung
    • 1
  • Cho-Rong Lee
    • 1
    • 2
  • Woo-Keun Song
    • 1
  • Youngsoo Jun
    • 1
    • 2
  • Sung-Gyoo Park
    • 1
    • 2
  1. 1.School of Life SciencesGwangju Institute of Science and Technology (GIST)GwangjuRepublic of Korea
  2. 2.Immune Synapse Research CenterGwangju Institute of Science and Technology (GIST)GwangjuRepublic of Korea

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