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Archives of Virology

, Volume 159, Issue 9, pp 2519–2521 | Cite as

A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA-2

  • D. F. Quito-Avila
  • M. A. Ibarra
  • R. Alvarez
  • E. L. Peralta
  • R. R. Martin
Annotated Sequence Record

Abstract

Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5’ terminus.

Keywords

Coat Protein Gene Movement Protein Black Raspberry Movement Protein Gene Canadian Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported in part by the Ecuadorean Science and Technology Secretariat (SENESCYT) through the PROMETEO program. The authors also thank blackberry growers in Paute-Azuay and Tisaleo-Tungurahua for granting us access to their fields.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • D. F. Quito-Avila
    • 1
  • M. A. Ibarra
    • 1
  • R. Alvarez
    • 1
  • E. L. Peralta
    • 1
  • R. R. Martin
    • 2
  1. 1.Centro de Investigaciones Biotecnologicas del Ecuador, Escuela Superior Politecnica del Litoral (CIBE-ESPOL)GuayaquilEcuador
  2. 2.USDA-ARS, Horticultural Crops Research UnitCorvallisUSA

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