A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA-2
- 236 Downloads
Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5’ terminus.
KeywordsCoat Protein Gene Movement Protein Black Raspberry Movement Protein Gene Canadian Isolate
This research was supported in part by the Ecuadorean Science and Technology Secretariat (SENESCYT) through the PROMETEO program. The authors also thank blackberry growers in Paute-Azuay and Tisaleo-Tungurahua for granting us access to their fields.
- 10.Murant AF, Mayo MA, Raschke JH (1986) Some biochemical properties of raspberry bushy dwarf virus. Acta Hortic 186:23–30Google Scholar
- 12.Quito-Avila DF, Peralta EL, Ibarra MA, Alvarez R, Ratti MF, Espinoza L, Martin RR (2013) First report of Raspberry bushy dwarf virus in blackberry in Ecuador. Plant Dis 97:1003Google Scholar