Archives of Virology

, Volume 159, Issue 8, pp 2141–2144 | Cite as

Allelic variants of PRNP in 16 Chinese local sheep breeds

  • Zouran Lan
  • Jinming Li
  • Chengyou Sun
  • Yutian Liu
  • Yonggang Zhao
  • Tianying Chi
  • Xiaojing Yu
  • Fangfang Song
  • Zhiliang WangEmail author
Brief Report


Here, polymorphisms of the ovine prion protein gene were analyzed in 486 Chinese sheep from 16 main local breeds. Polymorphisms R or H at codons 154 and four polymorphisms at codon 171 encoding Q, R, H, or K were identified. The A/V polymorphism at codon 136 was not observed, and all sheep were homozygous for A at this position. In addition, ten polymorphisms at codons 21, 101, 112, 127, 138, 141, 143, 146, 153 and 189 were detected. The predominant Q allele occurred at codon 171, with a high frequency of 88.68 %, implying a risk of scrapie in China.


Bovine Spongiform Encephalopathy Scrapie Sheep Breed Chronic Wasting Disease PRNP Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the China National Transgenic Major Projects (No. 2009ZX08008-011B) and China National Department Public Benefit Research Foundation (No. 200903037).


  1. 1.
    Palmer MS, Dryden AJ, Hughes JT, Collinge J (1991) Homozygous prion protein genotype predisposes to sporadic Creutzfeldt-Jakob disease. Nature 352(6333):340–342. doi: 10.1038/352340a0 PubMedCrossRefGoogle Scholar
  2. 2.
    Mead S, Stumpf MP, Whitfield J, Beck JA, Poulter M, Campbell T, Uphill JB, Goldstein D, Alpers M, Fisher EM, Collinge J (2003) Balancing selection at the prion protein gene consistent with prehistoric kurulike epidemics. Science 300(5619):640–643. doi: 10.1126/science.1083320 PubMedCrossRefGoogle Scholar
  3. 3.
    Goldmann W, Martin T, Foster J, Hughes S, Smith G, Hughes K, Dawson M, Hunter N (1996) Novel polymorphisms in the caprine PrP gene: a codon 142 mutation associated with scrapie incubation period. J Gener Virol 77(Pt 11):2885–2891CrossRefGoogle Scholar
  4. 4.
    Moore RC, Hope J, McBride PA, McConnell I, Selfridge J, Melton DW, Manson JC (1998) Mice with gene targetted prion protein alterations show that Prnp, Sinc and Prni are congruent. Nat Genet 18(2):118–125. doi: 10.1038/ng0298-118 PubMedCrossRefGoogle Scholar
  5. 5.
    Heaton MP, Leymaster KA, Freking BA, Hawk DA, Smith TP, Keele JW, Snelling WM, Fox JM, Chitko-McKown CG, Laegreid WW (2003) Prion gene sequence variation within diverse groups of U.S. sheep, beef cattle, and deer. Mamm Genome: Off J Int Mamm Genome Soc 14(11):765–777. doi: 10.1007/s00335-003-2283-y
  6. 6.
    Belt PB, Muileman IH, Schreuder BE, Bos-de Ruijter J, Gielkens AL, Smits MA (1995) Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. J Gen Virol 76(Pt 3):509–517PubMedCrossRefGoogle Scholar
  7. 7.
    Billinis C, Psychas V, Leontides L, Spyrou V, Argyroudis S, Vlemmas I, Leontides S, Sklaviadis T, Papadopoulos O (2004) Prion protein gene polymorphisms in healthy and scrapie-affected sheep in Greece. J Gen Virol 85(Pt 2):547–554PubMedCrossRefGoogle Scholar
  8. 8.
    Zhang L, Li N, Fan B, Fang M, Xu W (2004) PRNP polymorphisms in Chinese ovine, caprine and bovine breeds. Anim Genet 35(6):457–461. doi: 10.1111/j.1365-2052.2004.01204.x PubMedCrossRefGoogle Scholar
  9. 9.
    Lan Z, Wang ZL, Liu Y, Zhang X (2006) Prion protein gene (PRNP) polymorphisms in Xinjiang local sheep breeds in China. Arch Virol 151(10):2095–2101. doi: 10.1007/s00705-006-0758-3 PubMedCrossRefGoogle Scholar
  10. 10.
    Thorgeirsdottir S, Sigurdarson S, Thorisson HM, Georgsson G, Palsdottir A (1999) PrP gene polymorphism and natural scrapie in Icelandic sheep. J Gen Virol 80(Pt 9):2527–2534PubMedGoogle Scholar
  11. 11.
    Prusiner SB, Scott MR, DeArmond SJ, Cohen FE (1998) Prion protein biology. Cell 93(3):337–348PubMedCrossRefGoogle Scholar
  12. 12.
    Elsen JM, Amigues Y, Schelcher F, Ducrocq V, Andreoletti O, Eychenne F, Khang JV, Poivey JP, Lantier F, Laplanche JL (1999) Genetic susceptibility and transmission factors in scrapie: detailed analysis of an epidemic in a closed flock of Romanov. Arch Virol 144(3):431–445PubMedCrossRefGoogle Scholar
  13. 13.
    Buschmann A, Biacabe AG, Ziegler U, Bencsik A, Madec JY, Erhardt G, Luhken G, Baron T, Groschup MH (2004) Atypical scrapie cases in Germany and France are identified by discrepant reaction patterns in BSE rapid tests. J Virol Methods 117(1):27–36. doi: 10.1016/j.jviromet.2003.11.017 PubMedCrossRefGoogle Scholar
  14. 14.
    Moum T, Olsaker I, Hopp P, Moldal T, Valheim M, Benestad SL (2005) Polymorphisms at codons 141 and 154 in the ovine prion protein gene are associated with scrapie Nor98 cases. J Gen Virol 86(Pt 1):231–235. doi: 10.1099/vir.0.80437-0 PubMedCrossRefGoogle Scholar
  15. 15.
    Acin C, Martin-Burriel I, Goldmann W, Lyahyai J, Monzon M, Bolea R, Smith A, Rodellar C, Badiola JJ, Zaragoza P (2004) Prion protein gene polymorphisms in healthy and scrapie-affected Spanish sheep. J Gen Virol 85(Pt 7):2103–2110. doi: 10.1099/vir.0.80047-0 PubMedCrossRefGoogle Scholar
  16. 16.
    O’Rourke KI, Holyoak GR, Clark WW, Mickelson JR, Wang S, Melco RP, Besser TE, Foote WC (1997) PrP genotypes and experimental scrapie in orally inoculated Suffolk sheep in the United States. J Gen Virol 78(Pt 4):975–978PubMedGoogle Scholar
  17. 17.
    Hunter N (1997) PrP genetics in sheep and the applications for scrapie and BSE. Trends Microbiol 5(8):331–334PubMedCrossRefGoogle Scholar
  18. 18.
    Goldmann W, Hunter N, Smith G, Foster J, Hope J (1994) PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie. J Gen Virol 75(Pt 5):989–995PubMedCrossRefGoogle Scholar
  19. 19.
    Hunter N, Foster JD, Goldmann W, Stear MJ, Hope J, Bostock C (1996) Natural scrapie in a closed flock of Cheviot sheep occurs only in specific PrP genotypes. Arch Virol 141(5):809–824PubMedCrossRefGoogle Scholar
  20. 20.
    Baylis M, Goldmann W, Houston F, Cairns D, Chong A, Ross A, Smith A, Hunter N, McLean AR (2002) Scrapie epidemic in a fully PrP-genotyped sheep flock. J Gen Virol 83(Pt 11):2907–2914PubMedGoogle Scholar
  21. 21.
    Hunter N, Moore L, Hosie BD, Dingwall WS, Greig A (1997) Association between natural scrapie and PrP genotype in a flock of Suffolk sheep in Scotland. Vet Rec 140(3):59–63PubMedCrossRefGoogle Scholar
  22. 22.
    Baylis M, Chihota C, Stevenson E, Goldmann W, Smith A, Sivam K, Tongue S, Gravenor MB (2004) Risk of scrapie in British sheep of different prion protein genotype. J Gen Virol 85(Pt 9):2735–2740. doi: 10.1099/vir.0.79876-0 PubMedCrossRefGoogle Scholar
  23. 23.
    Goldmann W, Baylis M, Chihota C, Stevenson E, Hunter N (2005) Frequencies of PrP gene haplotypes in British sheep flocks and the implications for breeding programmes. J Appl Microbiol 98(6):1294–1302. doi: 10.1111/j.1365-2672.2005.02568.x PubMedCrossRefGoogle Scholar
  24. 24.
    DeSilva U, Guo X, Kupfer DM, Fernando SC, Pillai AT, Najar FZ, So S, Fitch GQ, Roe BA (2003) Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep. Cytogenet Genome Res 102(1–4):89–94PubMedCrossRefGoogle Scholar
  25. 25.
    Dawson M, Hoinville LJ, Hosie BD, Hunter N (1998) Guidance on the use of PrP genotyping as an aid to the control of clinical scrapie. Scrapie Information Group. Vet Rec 142(23):623–625PubMedGoogle Scholar
  26. 26.
    Schreuder BE, van Keulen LJ, Smits MA, Langeveld JP, Stegeman JA (1997) Control of scrapie eventually possible? Vet Q 19(3):105–113. doi: 10.1080/01652176.1997.9694752 PubMedCrossRefGoogle Scholar
  27. 27.
    Arnold M, Meek C, Webb CR, Hoinville LJ (2002) Assessing the efficacy of a ram-genotyping programme to reduce susceptibility to scrapie in Great Britain. Prev Vet Med 56(3):227–249PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Zouran Lan
    • 1
    • 2
  • Jinming Li
    • 1
  • Chengyou Sun
    • 1
  • Yutian Liu
    • 1
  • Yonggang Zhao
    • 1
  • Tianying Chi
    • 1
  • Xiaojing Yu
    • 1
  • Fangfang Song
    • 1
  • Zhiliang Wang
    • 1
    Email author
  1. 1.China Animal Health and Epidemiology CenterQingdaoPeople’s Republic of China
  2. 2.Shandong Center for Animal Disease Control and PreventionJinanPeople’s Republic of China

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