Archives of Virology

, Volume 159, Issue 8, pp 2059–2067 | Cite as

Molecular characterization of three ferret badger (Melogale moschata) rabies virus isolates from Jiangxi province, China

  • Jinghui Zhao
  • Ye Liu
  • Shoufeng Zhang
  • Fei Zhang
  • Ying Wang
  • Lijuan Mi
  • Shuchao Wang
  • Rongliang Hu
Original Article
First Online:
Received:
Accepted:

Abstract

Ferret badger (FB) rabies viruses JX09-17(fb), JX09-18 and JX10-37 were isolated from three different regions in Jiangxi province, China, in 2009 and 2010. The complete nucleotide sequence identity between these three isolates was 87–93 %. Compared with the other Chinese rabies virus isolates and vaccine strains, 101 substitutions (53 in JX10-37, 23 in JX09-17(fb) and 25 in JX09-18) in the five structural proteins were observed, and 47 of these substitutions (27 in JX10-37, 14 in JX09-17(fb) and 6 in JX09-18) were unique among lyssaviruses. Amino acid substitutions of S231 and Q333 were noted respectively in the G protein antigenic site I of JX10-37 and site III in JX09-17(fb). Phylogenetic analysis showed that JX09-17(fb) is rooted within the China I lineage, JX09-18 is in China II, and JX10-37 is independent. Evolutionary analysis and comparative sequence data indicate that isolate JX10-37 is a variant virus that diverged from canine rabies viruses around 1933 (range 1886–1963).

Keywords

Rabies Vaccine Strain Rabies Virus Jiangxi Province Poyang Lake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This investigation was funded by the National Natural Science Foundation of China (30630049, 30972199) and the China National “973” Program (2005CB52300, 2011CB500705).

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

705_2014_2044_MOESM1_ESM.doc (40 kb)
Supplementary Table S1. Comparison of genome structures (nt) (DOC 39 kb)
705_2014_2044_MOESM2_ESM.doc (75 kb)
Supplementary Table S2. Virus sequences used in this study (DOC 75 kb)
705_2014_2044_MOESM3_ESM.doc (41 kb)
Supplementary Table S3. Primers used in this study (DOC 41 kb)
705_2014_2044_MOESM4_ESM.jpg (652 kb)
Supplementary Fig. S1 N protein amino acid alignment. Dots represent identity to CTN181; dashes are gaps for optimal alignment. R, RNA-binding domain (298-352); III, antigenic site III (313-337); I, antigenic site I (358-367); underline, antigenic site IV (359-366 and 375-383); B1, B-cell epitope (369-383); B2/T, B-cell epitope (404-418) and Th epitope (404-418); T, T-cell epitope (373-395). Boxes with discontinuous blue lines indicate substitutions unique to FB isolates. (JPEG 651 kb)
705_2014_2044_MOESM5_ESM.jpg (774 kb)
Supplementary Fig. S2 G protein amino acid alignment. Dots represent identity to CTN181; dashes are gaps for optimal alignment. Boxes with continuous red lines indicate antigenic sites in the following order: antigenic site II (34–42 and 198–200), antigenic site I (231), IV (264), III (330–338), and “a” (342). Boxes with discontinuous blue lines indicate substitutions unique to FB isolates. (JPEG 774 kb)
705_2014_2044_MOESM6_ESM.jpg (728 kb)
Supplementary Fig. S3 Multiple amino acid sequence alignment of the L protein. The dashed boxes indicate the six domains (I–VI). Three putative functional regions are indicated as follows: A, RNA-binding region; B, active site of polymerase; C, glycine-rich motif. Boxes with discontinuous blue lines indicate substitutions unique to FB isolates. (JPEG 727 kb)
705_2014_2044_MOESM7_ESM.jpg (429 kb)
Supplementary Fig. S4 Maximum clade credibility (MCC) tree from Bayesian coalescent analysis based on the full N gene sequences (1,353 nt). The estimated TMRCA for this dataset and its 95% HPD values are indicated. Each strain name is followed by the GenBank accession number, country of origin, host, and year of detection. The horizontal axis represents the time scale in years starting from 2010. Numbers at the main nodes represent posterior probability values. Red circles indicate FB isolates from this study. (JPEG 429 kb)

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Jinghui Zhao
    • 1
  • Ye Liu
    • 1
  • Shoufeng Zhang
    • 1
  • Fei Zhang
    • 1
  • Ying Wang
    • 1
  • Lijuan Mi
    • 1
  • Shuchao Wang
    • 1
  • Rongliang Hu
    • 1
  1. 1.Laboratory of Epidemiology and Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research InstituteAcademy of Military Medical SciencesChangchunPeople’s Republic of China

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