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Archives of Virology

, Volume 159, Issue 8, pp 2033–2041 | Cite as

An influenza A virus hemagglutinin (HA) epitope inserted in and expressed from several loci of the infectious bursal disease virus genome induces HA-specific antibodies

  • Yung-Yi C. Mosley
  • Ching Ching Wu
  • Tsang Long Lin
Original Article

Abstract

The N-terminus of infectious bursal disease virus (IBDV) VP5 has been shown to be capable of tolerating the insertion of small epitopes. The objective of the present study was to determine if IBDV genomic sites, including the 5’ end of vp5, could carry an influenza A virus hemagglutinin (HA) epitope. HA-expressing IBDVs were generated when the HA epitope was fused to the N-terminus of VP5 (HA5-IBDV) or VP4 (HA4-IBDV) or the C-terminus of VP1 (1HA-IBDV). Viral titers obtained after co-transfection with cDNA from the ha-containing segment and the complementary genomic segment were 1.3 × 104, 3.7 × 103 and 3.8 × 104 pfu/ml for HA5-IBDV, HA4-IBDV and 1HA-IBDV, respectively. The HA tag expression remained stable after 10 passages when the tag gene was inserted into the vp4 and vp1 genes. HA-IBDVs did not cause pathogenicity in specific-pathogen-free (SPF) chickens. However, only HA4-IBDV and 1HA-IBDV induced HA-specific antibodies, which were measured by ELISA with a maximum optical density (OD) value of 0.701 and 0.769, respectively, at 24 days after infection. Thus, IBDV can potentially be employed as a bivalent viral vector when the epitope is fused with VP4 or VP1.

Keywords

Antibody Titer Infectious Bursal Disease Virus Infectious Pancreatic Necrosis Virus Infectious Bursal Disease Virus Strain Infectious Bursal Disease Virus Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Debby Sherman for her assistance with electron microscopy. We also thank Bong-Suk Kim, Yi-Ning Chen, Chih-Chun Lee, Chun-Yu Tung, Wan-Jung Chen and Ching Giap Tan for their help with the animal study.

Supplementary material

705_2014_2036_MOESM1_ESM.doc (1.2 mb)
Supplementary material 1 (DOC 1223 kb)

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Yung-Yi C. Mosley
    • 1
  • Ching Ching Wu
    • 1
  • Tsang Long Lin
    • 1
  1. 1.Department of Comparative Pathobiology, College of Veterinary MedicinePurdue UniversityWest LafayetteUSA

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