Archives of Virology

, Volume 159, Issue 8, pp 1951–1960 | Cite as

Comparing the effect of Toll-like receptor agonist adjuvants on the efficiency of a DNA vaccine

  • Azadeh Sajadian
  • Alijan Tabarraei
  • Hoorieh Soleimanjahi
  • Fatemeh Fotouhi
  • Ali Gorji
  • Amir GhaemiEmail author
Original Article


We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were inoculated with 2 × 105 TC-1 cells, and they were then immunized with HPV-16 E7 DNA vaccine alone or with 50 μg of resiquimod or poly(I:C) individually. We found that poly(I:C) and resiquimod could induce more antigen-specific lymphocyte proliferation and cytolytic activity compared to vaccination with E7 DNA alone. While E7 DNA had no significant inhibitory effect on tumor growth, co-administration of poly(I:C) and resiquimod with E7 DNA induced significant tumor regression. Peripheral and local cytokine assays demonstrated that co-administration of poly(I:C) and resiquimod with E7 DNA induced circulating antigen-specific IFN-γ and nonspecific intratumoral IL-12. TLR3 and TLR7 agonists can be used to enhance the immune response to DNA vaccine immunogens. Taken together, these data indicate that combined vaccination with DNA encoding HPV-16 E7 plus TLR agonists provides a strategy for improving the efficacy of a vaccine as a possible immunotherapeutic strategy for cervical cancer.


Human Papilloma Virus Imiquimod Vaccine Adjuvant Resiquimod Tumor Treatment Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Human papilloma virus


Toll-like receptor




Antigen-presenting cell


Cytolytic T lymphocyte


Dendritic cell


Food and Drug Administration

IFN- γ

Interferon γ


Interleukin 4


Interleukin 10


Interleukin 12


Phenylmethanesulfonyl fluoride


Lactate dehydrogenase


3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide


Optical density


Fetal calf serum


1640 Roswell Park Memorial Institute (name of the medium)


T helper



This study was supported by Golestan University of Medical Sciences, Gorgan, Iran.


All the authors declare that they have no conflicting interests.


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Azadeh Sajadian
    • 1
  • Alijan Tabarraei
    • 2
  • Hoorieh Soleimanjahi
    • 3
  • Fatemeh Fotouhi
    • 4
  • Ali Gorji
    • 1
    • 5
    • 6
    • 7
  • Amir Ghaemi
    • 2
    • 8
    Email author
  1. 1.Shefa Neuroscience Research CenterTehranIran
  2. 2.Department of MicrobiologyGolestan University of Medical SciencesGorganIran
  3. 3.Department of Virology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  4. 4.Department of VirologyPasteur Institute of IranTehranIran
  5. 5.Institut für Physiologie I, Westfälische Wilhelms-Universität MünsterMünsterGermany
  6. 6.Klinik und Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität MünsterMünsterGermany
  7. 7.Department of neurologyWestfälische Wilhelms-Universität MünsterMünsterGermany
  8. 8.Shefa Neuroscience Research Center, Khatam Al-Anbiya HospitalTehranIran

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