Archives of Virology

, Volume 159, Issue 8, pp 2157–2160 | Cite as

A unique mitovirus from Glomeromycota, the phylum of arbuscular mycorrhizal fungi

  • Ryoko Kitahara
  • Yoji Ikeda
  • Hanako Shimura
  • Chikara Masuta
  • Tatsuhiro EzawaEmail author
Annotated Sequence Record


Arbuscular mycorrhizal (AM) fungi that belong to the phylum Glomeromycota associate with most land plants and supply mineral nutrients to the host plants. One of the four viral segments found by deep-sequencing of dsRNA in the AM fungus Rhizophagus clarus strain RF1 showed similarity to mitoviruses and is characterized in this report. The genome segment is 2,895 nucleotides in length, and the largest ORF was predicted by applying either the mold mitochondrial or the universal genetic code. The ORF encodes a polypeptide of 820 amino acids with a molecular mass of 91.2 kDa and conserves the domain of the mitovirus RdRp superfamily. Accordingly, the dsRNA was designated as R. clarus mitovirus 1 strain RF1 (RcMV1-RF1). Mitoviruses are localized exclusively in mitochondria and thus generally employ the mold mitochondrial genetic code. The distinct codon usage of RcMV1-RF1, however, suggests that the virus is potentially able to replicate not only in mitochondria but also in the cytoplasm. RcMV1-RF1 RdRp showed the highest similarity to the putative RdRp of a mitovirus-like ssRNA found in another AM fungus, followed by RdRp of a mitovirus in an ascomycotan ectomycorrhizal fungus. The three mitoviruses found in the three mycorrhizal fungi formed a deeply branching clade that is distinct from the two major clades in the genus Mitovirus.


Arbuscular Mycorrhizal Mycorrhizal Fungus Tryptophan Residue Universal Genetic Code Hypovirulent Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was partially supported by The Asahi Glass Foundation (CM and TE) and a Grant-in-Aid for Scientific Research (22380042) from Japan Society for the Promotion of Science (TE).

Supplementary material

705_2014_1999_MOESM1_ESM.pdf (2.5 mb)
Supplementary material 1 (PDF 2560 kb)


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Ryoko Kitahara
    • 1
  • Yoji Ikeda
    • 1
  • Hanako Shimura
    • 1
  • Chikara Masuta
    • 1
  • Tatsuhiro Ezawa
    • 1
    Email author
  1. 1.Graduate School of AgricultureHokkaido UniversitySapporoJapan

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